Laboratorio de Virología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
Mem Inst Oswaldo Cruz. 2010 Mar;105(2):144-8. doi: 10.1590/s0074-02762010000200006.
Infection with some genotypes of human papillomavirus (HPV) is the most important risk factor associated with cervical cancer (CC). Throughout the world, HPV type 58 prevalence varies from one region to another; it is higher in women from certain countries in Asia and Latin America, such as China and Mexico. Although intratypic variants have been reported on a few occasions, our knowledge about HPV 58 genetic variation remains limited. Therefore, this work aims to (i) determine the prevalence of HPV type 58 amongst Mexican women with invasive CC or precursor lesions and (ii) identify HPV 58 sequence variants. One hundred and forty five colposcopy clinic patients were studied. Genotyping of HPV 16, 18 and 58 was determined by specific nested PCR and HPV 58 variants were detected by direct sequencing. The general prevalence of HPV was 51.7% (75/145). HPV 16 was found in 30.6% (23/75) and HPV 58 in 24% (18/75) of the patients. HPV 18 was not identified in patients with cervical intraepithelial neoplasia (CIN) grade I; it was only found in those with CIN II, with a prevalence of 6.8% (3/44). In patients with CC, the prevalence of HPV 16 and 58 was 78.9%. Regarding HPV 58 variants, 94.4% of the HPV 58 sequences were identical to the prototype strain, whereas one sample showed changes at a single nucleotide. This study demonstrates a high prevalence of HPV 58 and a low genetic variability of E6 sequences amongst Mexican colposcopy patients.
某些型别的人乳头瘤病毒（HPV）感染是与宫颈癌（CC）相关的最重要危险因素。在全球范围内，HPV 58 型的流行率因地区而异；在中国和墨西哥等亚洲和拉丁美洲的某些国家，女性中 HPV 58 型的流行率更高。尽管已经报道了几次同型内变异，但我们对 HPV 58 基因变异的了解仍然有限。因此，这项工作旨在：（i）确定患有浸润性 CC 或前病变的墨西哥女性中 HPV 58 型的流行率；（ii）鉴定 HPV 58 序列变异体。对 145 名阴道镜诊所患者进行了研究。通过特异性巢式 PCR 确定 HPV 16、18 和 58 的基因型，并通过直接测序检测 HPV 58 变异体。HPV 的总流行率为 51.7%（75/145）。在 75 名患者中发现 HPV 16 占 30.6%（23/75），HPV 58 占 24%（18/75）。HPV 18 未在 CIN I 级患者中检出；仅在 CIN II 患者中发现，其流行率为 6.8%（3/44）。在 CC 患者中，HPV 16 和 58 的流行率为 78.9%。关于 HPV 58 变异体，94.4%的 HPV 58 序列与原型株相同，而一个样本在单个核苷酸处发生了变化。本研究表明，墨西哥阴道镜患者中 HPV 58 的流行率较高，E6 序列的遗传变异性较低。
