Lee Hyun Kyoung, Lee Byoung Hee, Seok Seung Hyeok, Baek Min Won, Lee Hui Young, Kim Dong Jae, Na Yi Rang, Noh Kyoung Jin, Park Sung Hoon, Kumar Dutta Noton, Kariwa Hiroaki, Nakauchi Mina, Heo Suk Jin, Park Jae Hak
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
J Vet Sci. 2010 Jun;11(2):165-7. doi: 10.4142/jvs.2010.11.2.165.
Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.
严重急性呼吸综合征（SARS）是一种危及生命的疾病，准确诊断至关重要。尽管已经开发出许多用于SARS诊断的工具，但由于与其他冠状病毒的交叉反应，阴性血清中可能会出现假阳性反应。我们使用重组形式的SARS病毒核衣壳蛋白制备了多克隆和单克隆抗体（Abs）。通过蛋白质印迹法测定了这些抗SARS抗体对人冠状病毒（HCoV）229E和HCoV OC43的交叉反应性。所产生的抗体与重组SARS病毒核衣壳蛋白发生反应，但与HCoV 229E或HCoV OC43不发生反应。
