饮食模式与动脉粥样硬化风险社区（ARIC）颈动脉 MRI 研究中流式细胞术测量的炎症和细胞活化生物标志物之间的关联。
Associations between dietary patterns and flow cytometry-measured biomarkers of inflammation and cellular activation in the Atherosclerosis Risk in Communities (ARIC) Carotid Artery MRI Study.
机构信息
Division of Epidemiology & Disease Control, The University of Texas Health Sciences Center School of Public Health, 1200 Herman Pressler, Suite E-641, Houston, TX 77030, USA.
出版信息
Atherosclerosis. 2010 Sep;212(1):260-7. doi: 10.1016/j.atherosclerosis.2010.04.026. Epub 2010 Apr 29.
BACKGROUND
Specific foods and overall dietary patterns are associated with soluble biomarkers of systemic inflammation and endothelial activation. However, no large epidemiological studies have evaluated relationships between such dietary factors and cell-specific markers of activation and inflammation as measured by flow cytometry.
METHODS
Cell aggregates and multiple platelet and leukocyte markers were quantified by flow cytometry in fresh whole blood from 1101 white adults participating in the Carotid Artery MRI Study, a subset of the larger Atherosclerosis Risk in Communities (ARIC) Study. Two dietary patterns ("Healthy" and "Western") were empirically derived via principal components analysis using data collected by food frequency questionnaire. Cross-sectional associations between dietary patterns and flow cytometry-measured biomarkers were evaluated, adjusting for demographics and lifestyle factors, including medications use.
RESULTS
After multivariable adjustment, monocyte lipopolysaccharide receptor (CD14), monocyte toll-like receptor-2, and platelet glycoprotein IIb (CD41) showed inverse associations with the Healthy dietary pattern (p=0.01, 0.04, and 0.01, respectively). In contrast, the Western dietary pattern was positively associated with CD41 and platelet-granulocyte aggregates (p=0.01 and 0.04, respectively). Independent of other dietary factors, alcohol consumption was inversely associated with levels of pan-leukocyte marker (CD45), P-selectin (CD62P) on PLA1 and on PLA2 platelets, and platelet-monocyte, platelet-granulocyte, and platelet-lymphocyte aggregates.
CONCLUSION
Dietary patterns and alcohol intake were each cross-sectionally associated with select markers of cellular activation and inflammation measured by flow cytometry. These data are consistent with the hypothesis that holistic measures of dietary intake are associated with inflammation.
背景
特定的食物和整体饮食模式与全身炎症和内皮细胞激活的可溶性生物标志物有关。然而，尚无大型流行病学研究评估这些饮食因素与通过流式细胞术测量的细胞特异性激活和炎症标志物之间的关系。
方法
1101 名白种成年人参与了颈动脉 MRI 研究，这是更大的动脉粥样硬化风险社区（ARIC）研究的一部分，从他们的新鲜全血中通过流式细胞术定量了细胞聚集体和多种血小板和白细胞标志物。通过使用食物频率问卷收集的数据，通过主成分分析经验性地得出了两种饮食模式（“健康”和“西方”）。使用多变量调整评估了饮食模式与流式细胞术测量的生物标志物之间的横断面关联，包括调整人口统计学和生活方式因素，包括药物使用。
结果
在多变量调整后，单核细胞内毒素受体（CD14）、单核细胞 toll 样受体 2 和血小板糖蛋白 IIb（CD41）与健康饮食模式呈负相关（p=0.01、0.04 和 0.01，分别）。相比之下，西方饮食模式与 CD41 和血小板-粒细胞聚集体呈正相关（p=0.01 和 0.04，分别）。独立于其他饮食因素，酒精摄入与全白细胞标志物（CD45）、PLA1 和 PLA2 血小板上的 P-选择素（CD62P）、血小板-单核细胞、血小板-粒细胞和血小板-淋巴细胞聚集体的水平呈负相关。
结论
饮食模式和酒精摄入与通过流式细胞术测量的特定细胞激活和炎症标志物呈横断面相关。这些数据与整体饮食摄入与炎症相关的假设一致。
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