Institute of Laser for Post Graduate Studies, University of Baghdad, Baghdad, Iraq.
Lasers Med Sci. 2010 Sep;25(5):743-8. doi: 10.1007/s10103-010-0803-z. Epub 2010 Jun 16.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital acquired infection throughout the world especially in wound and burn infections, pneumonia, septicaemia and endocarditis. We describe the effect of a HeNe laser in combination with a TBO dye on the viability of MRSA. A total of 34 isolates of S. aureus were obtained from 100 patients suffering from burns or wounds and from the nasal vestibulum of medical and nonmedical staff as carriers; eight isolates were methicillin-resistant. The isolates were exposed for 5, 10 and 15 min to a HeNe laser at a wavelength of 632.8 nm and 7.5 mW output power in the presence of 50 microg/ml toluidune blue O photosensitizer. The viable count was substantially decreased as determined by the plate count method for the three exposure times, with 100% killing with the 15-min exposure time. No significant effect was observed on MRSA isolates exposed to the laser alone. So MRSA was completely eradicated following 15 min exposure to a 632.8-nm HeNe laser in the presence of 50 microg/ml toluidune blue O photosensitizer under in vitro conditions.
耐甲氧西林金黄色葡萄球菌（MRSA）是全世界医院获得性感染的主要原因，特别是在伤口和烧伤感染、肺炎、败血症和心内膜炎中。我们描述了氦氖激光与 TBO 染料联合对耐甲氧西林金黄色葡萄球菌活力的影响。从 100 名患有烧伤或伤口的患者和医务人员及非医务人员的鼻腔前庭中获得了总共 34 株金黄色葡萄球菌分离株，其中 8 株为耐甲氧西林金黄色葡萄球菌。将分离株在波长为 632.8nm 和 7.5mW 输出功率的氦氖激光下暴露 5、10 和 15 分钟，同时存在 50μg/ml 的甲苯胺蓝 O 光敏剂。通过平板计数法确定，三种暴露时间均使活菌数显著减少，15 分钟的暴露时间可达到 100%的杀灭率。单独用激光照射耐甲氧西林金黄色葡萄球菌分离株时，没有观察到明显的效果。因此，在体外条件下，当存在 50μg/ml 的甲苯胺蓝 O 光敏剂时，耐甲氧西林金黄色葡萄球菌在 632.8nm 氦氖激光下照射 15 分钟后被完全根除。
