Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Macromol Biosci. 2010 Nov 10;10(11):1330-7. doi: 10.1002/mabi.201000165.
Polymer scaffolds that direct elongation and orientation of cultured cells can enable tissue engineered muscle to act as a mechanically functional unit. We combined micromolding and microablation technologies to create muscle tissue engineering scaffolds from the biodegradable elastomer poly(glycerol sebacate). These scaffolds exhibited well defined surface patterns and pores and robust elastomeric tensile mechanical properties. Cultured C2C12 muscle cells penetrated the pores to form spatially controlled engineered tissues. Scanning electron and confocal microscopy revealed muscle cell orientation in a preferential direction, parallel to micromolded gratings and long axes of microablated anisotropic pores, with significant individual and interactive effects of gratings and pore design.
聚合物支架可以引导培养细胞的伸长和取向，从而使组织工程肌肉能够作为机械功能单元发挥作用。我们结合微成型和微刻蚀技术，从可生物降解弹性体聚（癸二酸甘油酯）中制造出肌肉组织工程支架。这些支架具有明确定义的表面图案和孔以及坚固的弹性拉伸力学性能。培养的 C2C12 肌肉细胞渗透到孔中，形成空间可控的工程组织。扫描电子显微镜和共聚焦显微镜显示，肌肉细胞沿优选方向取向，与微成型光栅和微刻蚀各向异性孔的长轴平行，光栅和孔设计具有显著的个体和交互作用。
