Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore.
Int Endod J. 2010 Oct;43(10):930-9. doi: 10.1111/j.1365-2591.2010.01771.x. Epub 2010 Aug 3.
To characterise the bioactivity of a novel carboxymethyl chitosan (CMCS) scaffold with and without incorporating mineral trioxide aggregate (MTA) in a tooth model.
Cross-linked CMCS scaffold (CaC) and MTA-coated CaC (CaMT) scaffold were prepared by freeze-drying. The bioactivity of the scaffolds was tested in vitro in four different mineralisation solutions (bulk system) and ex vivo in simulated body fluid (SBF) in the tooth model. After mineralisation, the mineral deposits on the scaffolds were analysed using scanning electron microscopy, energy dispersive X-ray, and inductively coupled plasma mass spectroscopy. All data were statistically analysed using the two-sample t-test (P < 0.05).
Hydroxyapatite (HAP) deposition was observed on CaC and CaMT scaffolds after 1 week of mineralisation in the tooth model and in the bulk system. The deposition was significantly higher (P < 0.05) on CaMT scaffold than that on CaC scaffold. The amount of HAP formed in the tooth model was significantly lower (P < 0.05) than that in the bulk solution.
The CMCS scaffolds are bioactive and capable of biomineralisation by forming HAP within a tooth model ex vivo. The bioactivity of the CMCS scaffold can be enhanced by incorporating MTA.
研究一种新型羧甲基壳聚糖（CMCS）支架在不添加和添加矿化三氧化物聚合体（MTA）的情况下在牙齿模型中的生物活性。
通过冷冻干燥制备交联 CMCS 支架（CaC）和 MTA 涂覆的 CaC（CaMT）支架。通过体外在四种不同的矿化溶液（整体系统）和在牙齿模型中的模拟体液（SBF）中进行体外实验来测试支架的生物活性。矿化后，使用扫描电子显微镜、能谱仪和电感耦合等离子体质谱仪分析支架上的矿物沉积物。使用两样本 t 检验对所有数据进行统计分析（P < 0.05）。
在牙齿模型和整体系统中，经过 1 周矿化后，CaC 和 CaMT 支架上观察到羟基磷灰石（HAP）沉积。在 CaMT 支架上的沉积明显高于 CaC 支架（P < 0.05）。在牙齿模型中形成的 HAP 量明显低于整体溶液（P < 0.05）。
CMCS 支架具有生物活性，能够通过在体外牙齿模型中形成 HAP 进行生物矿化。通过添加 MTA 可以增强 CMCS 支架的生物活性。
