Biomedicine and Sports Science Research Group, School of Life Sciences, Edinburgh Napier University, 10 Colinton Road, Edinburgh, EH10 5DT, UK.
Eur J Appl Physiol. 2010 Dec;110(6):1259-68. doi: 10.1007/s00421-010-1616-2. Epub 2010 Aug 27.
Acute exercise alters the surface expression of toll-like receptors (TLRs) and HLA.DR on blood monocytes, which could transiently compromise immunity. As serum factors might be responsible, we examined the effects of autologous post-exercise serum exposure on TLR2, TLR4 and HLA.DR expression on resting blood monocytes and their subtypes. Eight trained cyclists completed an ergometer 60 km time trial. PBMCs and serum were obtained before, immediately after and 1 h after exercise. TLR2, TLR4 or HLA.DR expression (gMFI) was determined on blood monocyte subtypes expressing combinations of CD14 and CD16 by flow cytometry, and on resting monocytes exposed to 50% autologous serum (pre, immediately after or 1 h after exercise) for 18 h in culture. Immediately after exercise, total monocyte expression of TLR2 and TLR4 increased by 41 and 27%, respectively, while HLA.DR expression was 39% lower than baseline. TLR2 and TLR4 was 53 and 84% greater 1 h after exercise, respectively, while HLA.DR was 48% lower. Changes in TLR2 and TLR4 expression occurred on the CD14(++bright)/CD16(+dim) monocyte subtype only, while HLA.DR expression changed on the CD14(+dim)/CD16(++bright) subtype. Serum did not affect monocyte TLR2 or TLR4 expression but 1 h post serum increased expression of HLA.DR on total monocytes and the CD14(+dim)/CD16(++bright) subtype, which was in contrast to the change observed at this time after exercise. We conclude that a bout of strenuous aerobic exercise alters the surface expression of TLR2, TLR4 and HLA.DR on blood monocytes and some of their subtypes, but these changes appear to be unrelated to blood serum factors.
急性运动改变了血液单核细胞表面 Toll 样受体 (TLR) 和 HLA.DR 的表达，这可能会暂时损害免疫力。由于血清因素可能是罪魁祸首，我们研究了运动后自体血清暴露对静息血液单核细胞及其亚型上 TLR2、TLR4 和 HLA.DR 表达的影响。八名训练有素的自行车手完成了一次 60 公里计时赛。在运动前、运动后立即和 1 小时后采集 PBMC 和血清。通过流式细胞术确定表达 CD14 和 CD16 组合的血液单核细胞亚型上 TLR2、TLR4 或 HLA.DR 的表达 (gMFI)，并在静息单核细胞上确定 50%自体血清 (运动前、运动后立即或 1 小时后) 暴露于 50%自体血清 18 小时的培养物中。运动后立即，TLR2 和 TLR4 的总单核细胞表达分别增加了 41%和 27%，而 HLA.DR 的表达比基线低 39%。运动后 1 小时，TLR2 和 TLR4 分别增加了 53%和 84%，而 HLA.DR 则降低了 48%。TLR2 和 TLR4 的表达变化仅发生在 CD14(++bright)/CD16(+dim)单核细胞亚型上，而 HLA.DR 的表达变化发生在 CD14(+dim)/CD16(++bright)亚型上。血清不影响单核细胞 TLR2 或 TLR4 的表达，但 1 小时后血清增加了总单核细胞和 CD14(+dim)/CD16(++bright)亚型上 HLA.DR 的表达，这与运动后此时观察到的变化相反。我们得出结论，剧烈的有氧运动改变了血液单核细胞及其某些亚型上 TLR2、TLR4 和 HLA.DR 的表面表达，但这些变化似乎与血液血清因素无关。
