通过无细胞合成活性酶对单纯疱疹病毒胸苷激酶的信使核糖核酸进行特性鉴定。
Characterization of the mRNA for herpes simplex virus thymidine kinase by cell-free synthesis of active enzyme.
作者信息
Cremer K, Bodemer M, Summers W C
出版信息
Nucleic Acids Res. 1978 Jul;5(7):2333-44. doi: 10.1093/nar/5.7.2333.
The cytoplasmic mRNA which codes for the herpes simplex virus specific thymidine kinase (TK) is polyadenylated and is 14.5s in size. This corresponds to an RNA of 1400 nucleotides. The TK polypeptide is about 42,000 daltons, which requires 1100 nucleotide. We conclude that the cytoplasmic mRNA is monocistronic. The reticulocyte lysate cell-free translation system synthesizes enzymatically active HSV-TK which can be assayed with high specificity and sensitivity by use of 125I-iododeoxycytidine as a substrate.
编码单纯疱疹病毒特异性胸苷激酶（TK）的细胞质信使核糖核酸（mRNA）是多聚腺苷酸化的，大小为14.5s。这相当于一个1400个核苷酸的核糖核酸。TK多肽约为42,000道尔顿，这需要1100个核苷酸。我们得出结论，细胞质mRNA是单顺反子的。网织红细胞裂解物无细胞翻译系统合成具有酶活性的单纯疱疹病毒胸苷激酶（HSV-TK），通过使用125I-碘脱氧胞苷作为底物，可以以高特异性和灵敏度对其进行检测。
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