Department of Bioengineering, Rice University, Houston, TX, USA.
Int J Nanomedicine. 2010 Aug 9;5:445-54. doi: 10.2147/ijn.s10881.
The goal of this study was to develop near-infrared (NIR) resonant gold-gold sulfide nanoparticles (GGS-NPs) as dual contrast and therapeutic agents for cancer management via multiphoton microscopy followed by higher intensity photoablation. We demonstrate that GGS-NPs exposed to a pulsed, NIR laser exhibit two-photon induced photoluminescence that can be utilized to visualize cancerous cells in vitro. When conjugated with anti-HER2 antibodies, these nanoparticles specifically bind SK-BR-3 breast carcinoma cells that over-express the HER2 receptor, enabling the cells to be imaged via multiphoton microscopy with an incident laser power of 1 mW. Higher excitation power (50 mW) could be employed to induce thermal damage to the cancerous cells, producing extensive membrane blebbing within seconds leading to cell death. GGS-NPs are ideal multifunctional agents for cancer management because they offer the ability to pinpoint precise treatment sites and perform subsequent thermal ablation in a single setting.
本研究旨在开发近红外（NIR）共振金-金硫化物纳米粒子（GGS-NPs）作为双重对比剂和治疗剂，通过多光子显微镜进行癌症管理，然后进行高强度光烧蚀。我们证明，暴露于脉冲近红外激光下的 GGS-NPs 表现出双光子诱导的光致发光，可用于体外可视化癌细胞。当与抗 HER2 抗体结合时，这些纳米粒子特异性结合过度表达 HER2 受体的 SK-BR-3 乳腺癌细胞，使细胞能够在 1 mW 的入射激光功率下通过多光子显微镜进行成像。更高的激发功率（50 mW）可用于诱导癌细胞的热损伤，在几秒钟内产生广泛的细胞膜起泡，导致细胞死亡。GGS-NPs 是癌症管理的理想多功能制剂，因为它们能够精确地定位治疗部位，并在单次治疗中进行随后的热烧蚀。
