包涵体：新概念。
Inclusion bodies: a new concept.
机构信息
CIBER en Bioingeniería, Biomateriales y Nanomedicina, Bellaterra, 08193 Barcelona, Spain.
出版信息
Microb Cell Fact. 2010 Nov 1;9:80. doi: 10.1186/1475-2859-9-80.
Abstract
In the last decades, the understanding of inclusion body biology and consequently, of their properties and potential biotechnological applications have dramatically changed. Therefore, the development of new purification protocols aimed to preserve those properties is becoming a pushing demand.
摘要
在过去几十年中，人们对包涵体生物学的理解以及由此产生的对其性质和潜在生物技术应用的理解发生了巨大变化。因此，开发旨在保留这些特性的新纯化方案成为一种迫切需求。
相似文献
1
Inclusion bodies: a new concept.包涵体：新概念。
Microb Cell Fact. 2010 Nov 1;9:80. doi: 10.1186/1475-2859-9-80.
2
High-throughput recovery of therapeutic proteins from the inclusion bodies of Escherichia coli: an overview.从大肠杆菌包涵体中高通量回收治疗性蛋白质：综述
Methods Mol Biol. 2005;308:155-61. doi: 10.1385/1-59259-922-2:155.
3
Isolation, solubilization, refolding, and chromatographic purification of human growth hormone from inclusion bodies of Escherichia coli cells: a case study.从大肠杆菌细胞包涵体中分离、溶解、复性及色谱纯化人生长激素：一个案例研究
Methods Mol Biol. 2005;308:163-76. doi: 10.1385/1-59259-922-2:163.
4
Bioprocessing of therapeutic proteins from the inclusion bodies of Escherichia coli.从大肠杆菌包涵体中进行治疗性蛋白质的生物加工。
Adv Biochem Eng Biotechnol. 2003;85:43-93. doi: 10.1007/3-540-36466-8_3.
5
Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli.利用多粘类芽孢杆菌 PoxB 作为融合伴侣在大肠杆菌中形成活性包涵体。
Anal Biochem. 2012 Jul 1;426(1):63-5. doi: 10.1016/j.ab.2012.04.002. Epub 2012 Apr 7.
6
Functional protein aggregates: just the tip of the iceberg.功能性蛋白质聚集体：只是冰山一角。
Nanomedicine (Lond). 2015;10(18):2881-91. doi: 10.2217/nnm.15.125. Epub 2015 Sep 15.
7
Microfluidic chips with multi-junctions: an advanced tool in recovering proteins from inclusion bodies.具有多通道的微流控芯片：从包涵体中回收蛋白质的先进工具。
Bioengineered. 2015;6(1):1-4. doi: 10.4161/21655979.2014.987022. Epub 2015 Jan 7.
8
Expression, Solubilization, Refolding and Final Purification of Recombinant Proteins as Expressed in the form of "Classical Inclusion Bodies" in .在 中以“经典包涵体”形式表达的重组蛋白的表达、溶解、复性和最终纯化。
Protein Pept Lett. 2021;28(2):122-130. doi: 10.2174/0929866527999200729182831.
9
[Solubilization Specificities Interferon beta-1b from Inclusion Bodies].[包涵体中β-1b干扰素的溶解特性]
Bioorg Khim. 2015 Jul-Aug;41(4):403-10. doi: 10.1134/s1068162015040159.
10
Refolding, purification, and activation of miniplasminogen and microplasminogen isolated from E. coli inclusion bodies.从大肠杆菌包涵体中分离出的微型纤溶酶原和微纤溶酶原的复性、纯化及激活
Protein Expr Purif. 2007 Apr;52(2):395-402. doi: 10.1016/j.pep.2006.10.012. Epub 2006 Oct 26.
引用本文的文献
1
State-of-the-art and novel approaches to mild solubilization of inclusion bodies.包涵体温和溶解的先进与新颖方法。
Front Bioeng Biotechnol. 2023 Jul 20;11:1249196. doi: 10.3389/fbioe.2023.1249196. eCollection 2023.
2
Implementation of a Design of Experiments to Improve Periplasmic Yield of Functional ScFv Antibodies in a Phage Display Platform.在噬菌体展示平台中实施实验设计以提高功能性单链抗体片段（scFv）抗体的周质产量。
Adv Pharm Bull. 2022 May;12(3):583-592. doi: 10.34172/apb.2022.061. Epub 2021 Jul 3.
3
Fundamental insights in early-stage inclusion body formation.早期包涵体形成的基本认识。
Microb Biotechnol. 2023 May;16(5):893-900. doi: 10.1111/1751-7915.14117. Epub 2022 Jul 13.
4
In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics.展示于蛋白质纳米颗粒上的GWH1抗菌肽的体内杀菌功效：抗生素的一种潜在替代品
Pharmaceutics. 2020 Dec 17;12(12):1217. doi: 10.3390/pharmaceutics12121217.
5
Optimization of Methods for the Production and Refolding of Biologically Active Disulfide Bond-Rich Antibody Fragments in Microbial Hosts.微生物宿主中具有生物活性的富含二硫键抗体片段的生产与重折叠方法的优化
Antibodies (Basel). 2020 Aug 5;9(3):39. doi: 10.3390/antib9030039.
6
Production of Active Recombinant Hyaluronidase Inclusion Bodies from in Bl21(DE3) and characterization by FT-IR Spectroscopy.在 BL21(DE3) 中生产活性重组透明质酸酶包涵体及其傅里叶变换红外光谱学表征。
Int J Mol Sci. 2020 May 29;21(11):3881. doi: 10.3390/ijms21113881.
7
Solubilization of Human Interferon β-1b Inclusion Body Proteins by Organic Solvents.用有机溶剂溶解人干扰素β-1b包涵体蛋白
Adv Pharm Bull. 2020 Jun;10(2):233-238. doi: 10.34172/apb.2020.027. Epub 2020 Feb 18.
8
Improvement of solubility and yield of recombinant protein expression in using a two-step system.利用两步系统提高重组蛋白在[具体表达体系未明确]中的溶解度和表达产量。
Res Pharm Sci. 2019 Oct 4;14(5):400-407. doi: 10.4103/1735-5362.268200. eCollection 2019 Oct.
9
Fish Red Blood Cells Modulate Immune Genes in Response to Bacterial Inclusion Bodies Made of TNFα and a G-VHSV Fragment.鱼类红细胞响应由 TNFα 和 G-VHSV 片段组成的细菌包含体，调节免疫基因。
Front Immunol. 2019 May 22;10:1055. doi: 10.3389/fimmu.2019.01055. eCollection 2019.
10
Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies.热诱导型大肠杆菌中 ESAT-6 抗原的重组生产：培养规模和温度对代谢反应、伴侣蛋白表达和包涵体结构的影响。
Cell Stress Chaperones. 2019 Jul;24(4):777-792. doi: 10.1007/s12192-019-01006-x. Epub 2019 Jun 4.
本文引用的文献
1
Isolation of cell-free bacterial inclusion bodies.无细胞细菌包含体的分离。
Microb Cell Fact. 2010 Sep 17;9:71. doi: 10.1186/1475-2859-9-71.
2
Isolation of biologically active nanomaterial (inclusion bodies) from bacterial cells.从细菌细胞中分离具有生物活性的纳米材料（包含体）。
Microb Cell Fact. 2010 Sep 10;9:66. doi: 10.1186/1475-2859-9-66.
3
Side effects of chaperone gene co-expression in recombinant protein production.伴侣蛋白共表达在重组蛋白生产中的副作用。
Microb Cell Fact. 2010 Sep 2;9:64. doi: 10.1186/1475-2859-9-64.
4
Nanotechnology, bionanotechnology and microbial cell factories.纳米技术、生物纳米技术和微生物细胞工厂。
Microb Cell Fact. 2010 Jul 5;9:53. doi: 10.1186/1475-2859-9-53.
5
The nanoscale properties of bacterial inclusion bodies and their effect on mammalian cell proliferation.细菌包含体的纳米级特性及其对哺乳动物细胞增殖的影响。
Biomaterials. 2010 Aug;31(22):5805-12. doi: 10.1016/j.biomaterials.2010.04.008. Epub 2010 May 8.
6
Tunable geometry of bacterial inclusion bodies as substrate materials for tissue engineering.可调节细菌包含体的几何形状作为组织工程的基质材料。
Nanotechnology. 2010 May 21;21(20):205101. doi: 10.1088/0957-4484/21/20/205101. Epub 2010 Apr 23.
7
Protein-engineered biomaterials: highly tunable tissue engineering scaffolds.蛋白质工程生物材料：高度可调的组织工程支架。
Tissue Eng Part B Rev. 2010 Jun;16(3):285-93. doi: 10.1089/ten.teb.2009.0591.
8
Nanotechnology for bone materials.纳米技术在骨材料中的应用。
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2009 May-Jun;1(3):336-51. doi: 10.1002/wnan.23.
9
Carrier-free immobilized enzymes for biocatalysis.无载体固定化酶在生物催化中的应用。
Biotechnol Lett. 2010 Mar;32(3):341-50. doi: 10.1007/s10529-009-0173-4. Epub 2009 Nov 27.
10
Targeting lectin activity into inclusion bodies for the characterisation of glycoproteins.将凝集素活性靶向至包涵体以用于糖蛋白的表征。
Mol Biosyst. 2009 Aug;5(8):819-21. doi: 10.1039/b900526a. Epub 2009 Jun 23.
Zotero 插件