猪肺中功能性不变自然杀伤 T 细胞调节气道高反应性：一种潜在的动物模型。
Functional invariant NKT cells in pig lungs regulate the airway hyperreactivity: a potential animal model.
机构信息
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, USA.
出版信息
J Clin Immunol. 2011 Apr;31(2):228-39. doi: 10.1007/s10875-010-9476-4. Epub 2010 Nov 2.
Abstract
Important roles played by invariant natural killer T (iNKT) cells in asthma pathogenesis have been demonstrated. We identified functional iNKT cells and CD1d molecules in pig lungs. Pig iNKT cells cultured in the presence of α-GalCer proliferated and secreted Th1 and Th2 cytokines. Like in other animal models, direct activation of pig lung iNKT cells using α-GalCer resulted in acute airway hyperreactivity (AHR). Clinically, acute AHR-induced pigs had increased respiratory rate, enhanced mucus secretion in the airways, fever, etc. In addition, we observed petechial hemorrhages, infiltration of CD4(+) cells, and increased Th2 cytokines in AHR-induced pig lungs. Ex vivo proliferated iNKT cells of asthma induced pigs in the presence of C-glycoside analogs of α-GalCer had predominant Th2 phenotype and secreted more of Th2 cytokine, IL-4. Thus, baby pigs may serve as a useful animal model to study iNKT cell-mediated AHR caused by various environmental and microbial CD1d-specific glycolipid antigens.
摘要
已证实不变自然杀伤 T(iNKT)细胞在哮喘发病机制中发挥重要作用。我们在猪肺中鉴定出功能性 iNKT 细胞和 CD1d 分子。在 α-GalCer 存在的情况下培养的猪 iNKT 细胞增殖并分泌 Th1 和 Th2 细胞因子。与其他动物模型一样，使用 α-GalCer 直接激活猪肺 iNKT 细胞会导致急性气道高反应性(AHR)。临床上，急性 AHR 诱导的猪呼吸频率增加，气道内黏液分泌增加，发热等。此外，我们观察到 AHR 诱导的猪肺中有瘀点样出血、CD4+细胞浸润和 Th2 细胞因子增加。在 C-糖苷类似物 α-GalCer 的存在下，哮喘诱导的猪离体增殖的 iNKT 细胞具有明显的 Th2 表型，并分泌更多的 Th2 细胞因子 IL-4。因此，仔猪可能成为研究各种环境和微生物 CD1d 特异性糖脂抗原引起的 iNKT 细胞介导的 AHR 的有用动物模型。
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