Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Biofouling. 2010 Nov;26(8):973-83. doi: 10.1080/08927014.2010.534552.
This report details the efficacy of nitric oxide (NO)-releasing xerogel surfaces composed of N-(6-aminohexyl)aminopropyl trimethoxysilane (AHAP3) and isobutyltrimethoxysilane (BTMOS) against Candida albicans adhesion, viability, and biofilm formation. A parallel plate flow cell assay was used to examine the effect of NO on planktonic fungal cells. Nitric oxide fluxes as low as 14 pmol cm(-2) s(-1) were sufficient to reduce fungal adhesion by ∼49% over the controls after 90 min. By utilizing a fluorescence live/dead assay and replicate plating, NO flux was determined to reduce fungal viability in a dose-dependent manner. The formation of C. albicans biofilms on NO-releasing xerogel-coated silicon rubber (SiR) coupons was impeded when compared to control (non-NO-releasing) and bare SiR surfaces. The synergistic efficacy of NO and silver sulfadiazine against adhered fungal cells and biofilms is reported with increased killing and biofilm inhibition over NO alone.
本报告详细介绍了由 N-(6-氨基己基)氨丙基三甲氧基硅烷 (AHAP3) 和异丁基三甲氧基硅烷 (BTMOS) 组成的释放一氧化氮 (NO) 的气凝胶表面在抑制白色念珠菌黏附、活力和生物膜形成方面的功效。采用平行板流动池测定法来研究 NO 对浮游真菌细胞的影响。结果表明，在 90 分钟后，NO 通量低至 14 pmol cm(-2) s(-1)，即可使真菌黏附减少约 49%。通过利用荧光死活检测和重复接种平板法，发现 NO 通量呈剂量依赖性降低真菌活力。与对照（非 NO 释放）和裸硅橡胶 (SiR) 表面相比，NO 释放气凝胶涂层硅橡胶 (SiR) 上白色念珠菌生物膜的形成受到抑制。据报道，NO 和磺胺嘧啶银联合使用对黏附的真菌细胞和生物膜具有协同功效，与单独使用 NO 相比，杀菌和抑制生物膜的效果更强。
