在中温条件下能够有效进行纤维素糖化的福氏志贺氏菌 G3 的分离和特性描述。
Isolation and characterization of Shigella flexneri G3, capable of effective cellulosic saccharification under mesophilic conditions.
机构信息
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
出版信息
Appl Environ Microbiol. 2011 Jan;77(2):517-23. doi: 10.1128/AEM.01230-10. Epub 2010 Nov 19.
Abstract
A novel Shigella strain (Shigella flexneri G3) showing high cellulolytic activity under mesophilic, anaerobic conditions was isolated and characterized. The bacterium is Gram negative, short rod shaped, and nonmotile and displays effective production of glucose, cellobiose, and other oligosaccharides from cellulose (Avicel PH-101) under optimal conditions (40°C and pH 6.5). Approximately 75% of the cellulose was hydrolyzed in modified ATCC 1191 medium containing 0.3% cellulose, and the oligosaccharide production yield and specific production rate reached 375 mg g Avicel(-1) and 6.25 mg g Avicel(-1) h(-1), respectively, after a 60-hour incubation. To our knowledge, this represents the highest oligosaccharide yield and specific rate from cellulose for mesophilic bacterial monocultures reported so far. The results demonstrate that S. flexneri G3 is capable of rapid conversion of cellulose to oligosaccharides, with potential biofuel applications under mesophilic conditions.
摘要
一株新型志贺氏菌（福氏志贺氏菌 G3）在中温、厌氧条件下表现出较高的纤维素分解活性，现已被分离并鉴定。该细菌呈革兰氏阴性、短杆状、非运动性，在最佳条件（40°C 和 pH6.5）下能有效产生葡萄糖、纤维二糖和其他纤维素寡糖（Avicel PH-101）。在含有 0.3%纤维素的改良 ATCC 1191 培养基中，约有 75%的纤维素被水解，60 小时培养后，寡糖产量和比生产速率分别达到 375mg g Avicel(-1)和 6.25mg g Avicel(-1) h(-1)。据我们所知，这是目前为止报道的中温细菌纯培养物从纤维素中获得的最高寡糖产量和比生产速率。结果表明，福氏志贺氏菌 G3 能够快速将纤维素转化为寡糖，在中温条件下具有潜在的生物燃料应用价值。
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