Biomolecular Mass Sepctrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
Proteomics Clin Appl. 2009 Jan;3(1):6-17. doi: 10.1002/prca.200800090. Epub 2008 Nov 20.
MS is an important analytical tool in clinical proteomics, primarily in the disease-specific discovery, identification and characterisation of proteomic biomarkers and patterns. MS-based proteomics is increasingly used in clinical validation and diagnostic method development. The latter departs from the typical application of MS-based proteomics by exchanging some of the high performance of analysis for the throughput, robustness and simplicity required for clinical diagnostics. Although conventional MS-based proteomics has become an important field in clinical applications, some of the most recent MS technologies have not yet been extensively applied in clinical proteomics. In this review, we will describe the current state of MS in clinical proteomics and look to the future of this field.
MS 是临床蛋白质组学中的一个重要分析工具，主要用于疾病特异性的蛋白质组生物标志物和模式的发现、鉴定和特征描述。基于 MS 的蛋白质组学越来越多地用于临床验证和诊断方法的开发。后者与基于 MS 的蛋白质组学的典型应用不同，它通过用临床诊断所需的通量、稳健性和简单性来替代部分分析的高性能。尽管传统的基于 MS 的蛋白质组学已成为临床应用中的一个重要领域，但一些最新的 MS 技术尚未在临床蛋白质组学中得到广泛应用。在这篇综述中，我们将描述 MS 在临床蛋白质组学中的现状，并展望该领域的未来。
