脂阿拉伯甘露聚糖用于结核病血清学诊断的评估。
Evaluation of lipoarabinomannan for the serological diagnosis of tuberculosis.
作者信息
Sada E, Brennan P J, Herrera T, Torres M
机构信息
Department of Microbiology, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico.
出版信息
J Clin Microbiol. 1990 Dec;28(12):2587-90. doi: 10.1128/jcm.28.12.2587-2590.1990.
Abstract
The availability of highly purified lipoarabinomannan from Mycobacterium tuberculosis in its native acylated, highly antigenic state allowed its application to the serodiagnosis of tuberculosis in patients from the Republic of Mexico. Antilipoarabinomannan immunoglobulin G antibodies in sera from 66 patients with pulmonary, miliary, and pleural tuberculosis and tuberculosis lymphadenities were measured by using the enzyme-linked immunosorbent assay against sera from a control population of healthy individuals, people with histoplasmosis, and people with lung diseases not caused by mycobacteria. The results pointed to an unexpectedly high degree of specificity of 91% and a sensitivity of 72%, comparable to figures from previous studies with other purified antigens; most of the false-positive results were for patients with histoplasmosis. Thus, lipoarabinomannan of M. tuberculosis is a potentially useful antigen for the serodiagnosis of tuberculosis.
摘要
来自结核分枝杆菌的高度纯化的脂阿拉伯甘露聚糖以其天然酰化、高抗原性状态的可得性，使其能够应用于墨西哥共和国患者的结核病血清诊断。通过酶联免疫吸附测定法，针对健康个体、组织胞浆菌病患者和非分枝杆菌引起的肺部疾病患者的对照人群血清，检测了66例患有肺结核、粟粒性肺结核、胸膜结核和结核性淋巴结炎患者血清中的抗脂阿拉伯甘露聚糖免疫球蛋白G抗体。结果显示出出乎意料的高特异性，为91%，敏感性为72%，与先前使用其他纯化抗原的研究结果相当；大多数假阳性结果出现在组织胞浆菌病患者中。因此，结核分枝杆菌的脂阿拉伯甘露聚糖是一种潜在有用的结核病血清诊断抗原。
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