Wong H C, Chao W L, Lee S J
Department of Microbiology, Soochow University, Taiwan, Republic of China.
Appl Environ Microbiol. 1990 Oct;56(10):3101-4. doi: 10.1128/aem.56.10.3101-3104.1990.
A variety of foods were examined for the incidence of Listeria monocytogenes, and the bacterial isolates were further characterized. L. monocytogenes was selected on LiCl-phenylethanol-moxalactam agar after enrichments and identified by several biochemical, mobility, and CAMP tests. L. monocytogenes was isolated from 58.8% of pork samples, 50% of chicken carcasses, 38% of turkey parts, 34% of frozen semiready foods, 24% of beef steaks, 12.2% of vegetables, 10.5% of seafoods, and 4.4% of frozen dim sum but was not found in the Chinese pickles and fermented milks. Isolates from seafoods, turkey parts, and beef samples had higher hemolytic activity than those from other samples. The isolates were highly susceptible to ampicillin, cephalothin, chloramphenicol, erythromycin, gentamicin, kanamycin, neomycin, novobiocin, penicillin, and streptomycin. About 14.5% of the isolates were resistant to methicillin, and 14.5% were resistant to tetracycline. The majority of the isolates from turkey parts and beef steaks were serotype 1, and those from chicken and pork samples were serotype 4 and others. Hemolytic activity, methicillin susceptibility, and serotype distribution of the isolates from domestic and imported food samples were significantly different. The results suggest the presence of food- or geography-specific L. monocytogenes strains.
对多种食品进行了单核细胞增生李斯特菌发生率的检测，并对分离出的细菌进行了进一步鉴定。单核细胞增生李斯特菌经富集培养后在含氯化锂-苯乙醇-莫西沙星的琼脂培养基上筛选，并通过多项生化、运动性和CAMP试验进行鉴定。单核细胞增生李斯特菌在58.8%的猪肉样本、50%的鸡胴体、38%的火鸡部位、34%的冷冻即食食品、24%的牛排、12.2%的蔬菜、10.5%的海产品和4.4%的冷冻点心样本中被分离出，但在中国泡菜和发酵乳中未发现。从海产品、火鸡部位和牛肉样本中分离出的菌株比其他样本中的菌株具有更高的溶血活性。这些分离菌株对氨苄西林、头孢噻吩、氯霉素、红霉素、庆大霉素、卡那霉素、新霉素、新生霉素、青霉素和链霉素高度敏感。约14.5%的分离菌株对甲氧西林耐药，14.5%对四环素耐药。从火鸡部位和牛排中分离出的大多数菌株为血清型1，而从鸡肉和猪肉样本中分离出的菌株为血清型4及其他血清型。国产和进口食品样本中分离菌株的溶血活性、对甲氧西林的敏感性和血清型分布存在显著差异。结果表明存在食品或地域特异性的单核细胞增生李斯特菌菌株。
