Smithsonian Marine Station at Ft. Pierce, 701 Seaway Drive, Ft. Pierce, Florida 34949, USA.
J Nat Prod. 2011 Apr 25;74(4):871-6. doi: 10.1021/np1008015. Epub 2011 Feb 22.
Malyngamide 3 (1) and cocosamides A (2) and B (3) were isolated from the lipophilic extract of a collection of Lyngbya majuscula from Cocos Lagoon, Guam. The planar structures of compounds 1-3 were determined by spectroscopic methods. The absolute configuration of 1 was determined by modified Mosher's method, NOESY data, and comparison with lyngbic acid (4). The absolute configurations of 2 and 3 were assigned by enantioselective HPLC analysis and comparison with the closely related compound pitipeptolide A (5). Compounds 1-3 showed weak cytotoxicity against MCF7 breast cancer and HT-29 colon cancer cells.
马蔺酰胺 3（1）、可可酰胺 A（2）和 B（3）从关岛可可泻湖大团扇藻的脂溶性提取物中分离得到。通过光谱方法确定了化合物 1-3 的平面结构。通过改进的莫舍尔法、NOESY 数据和与石杉碱甲（4）的比较确定了化合物 1 的绝对构型。通过对映体选择性 HPLC 分析和与密切相关的化合物 pitipeptolide A（5）的比较确定了化合物 2 和 3 的绝对构型。化合物 1-3 对 MCF7 乳腺癌和 HT-29 结肠癌细胞表现出较弱的细胞毒性。
