University of Illinois at Urbana-Champaign, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science & Technology, Urbana, Illinois 61801, USA.
J Biomed Opt. 2011 Feb;16(2):026019. doi: 10.1117/1.3549204.
We show that applying the Laplace operator to a speckle-free quantitative phase image reveals an unprecedented level of detail in cell structure, without the gradient artifacts associated with differential interference contrast microscopy, or photobleaching and phototoxicity limitations common in fluorescence microscopy. This method, referred to as Laplace phase microscopy, is an efficient tool for tracking vesicles and organelles in living cells. The principle is demonstrated by tracking organelles in cardiomyocytes and vesicles in neurites of hippocampal neurons, which to our knowledge are the first label-free diffusion measurements of the organelles in such cells.
我们表明，对无散斑的定量相位图像应用拉普拉斯算子，可以揭示出细胞结构前所未有的细节水平，而不会出现微分干涉对比显微镜相关的梯度伪影，也不会出现荧光显微镜中常见的漂白和光毒性限制。这种方法称为拉普拉斯相位显微镜，是跟踪活细胞中的囊泡和细胞器的有效工具。该原理通过在心肌细胞中的细胞器和海马神经元突中的囊泡进行跟踪来证明，据我们所知，这是这些细胞中细胞器的首次无标记扩散测量。
