Wang Yiran, Huang Weidong, Sathitsuksanoh Noppadon, Zhu Zhiguang, Zhang Y-H Percival
Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061, USA.
Chem Biol. 2011 Mar 25;18(3):372-80. doi: 10.1016/j.chembiol.2010.12.019.
Different from NAD(P)H regeneration approaches mediated by a single enzyme or a whole-cell microorganism, we demonstrate high-yield generation of NAD(P)H from a renewable biomass sugar--cellobiose through in vitro synthetic enzymatic pathways consisting of 12 purified enzymes and coenzymes. When the NAD(P)H generation system was coupled with its consumption reaction mediated by xylose reductase, the NADPH yield was as high as 11.4 mol NADPH per cellobiose (i.e., 95% of theoretical yield--12 NADPH per glucose unit) in a batch reaction. Consolidation of endothermic reactions and exothermic reactions in one pot results in a very high energy-retaining efficiency of 99.6% from xylose and cellobiose to xylitol. The combination of this high-yield and projected low-cost biohydrogenation and aqueous phase reforming may be important for the production of sulfur-free liquid jet fuel in the future.
与由单一酶或全细胞微生物介导的NAD(P)H再生方法不同，我们通过由12种纯化酶和辅酶组成的体外合成酶途径，证明了可再生生物质糖——纤维二糖能高产NAD(P)H。当NAD(P)H生成系统与其由木糖还原酶介导的消耗反应偶联时，在分批反应中，NADPH产量高达每纤维二糖11.4摩尔NADPH（即理论产量的95%——每葡萄糖单位12个NADPH）。将吸热反应和放热反应合并在一个反应釜中，使得从木糖和纤维二糖到木糖醇的能量保留效率非常高，达到99.6%。这种高产且预计低成本的生物氢化和水相重整相结合，可能对未来生产无硫液体喷气燃料具有重要意义。
