CESAM & Department of Biology, University of Aveiro, Aveiro, Portugal.
Nanotoxicology. 2012 Mar;6(2):134-43. doi: 10.3109/17435390.2011.562327. Epub 2011 Mar 29.
In the present study, the main goal was to compare the effects of ionic copper versus copper nanoparticles in Enchytraeus albidus assessing the effect at the biomarker level, testing different concentrations and exposure times. Measured parameters were lipid peroxidation (LPO), total, reduced and oxidized glutathione content (TG, GSH and GSSG), the enzymatic activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione S-transferases (GSTs) and cholinesterases (ChEs). Results showed that both salt- and nano-copper caused oxidative stress and damage to E. albidus, as confirmed by LPO levels, and effects could be discriminated between the copper forms. Nevertheless and despite the visible discrimination between nano and the salt form (time and exposure dependent), there was no single or a set of biomarkers that provided the best discrimination.
在本研究中，主要目标是比较离子态铜与铜纳米粒子对白色盲颤蚓（Enchytraeus albidus）的影响，在生物标志物水平上测试不同浓度和暴露时间的影响。测量的参数包括脂质过氧化 (LPO)、总、还原和氧化型谷胱甘肽含量 (TG、GSH 和 GSSG)、超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽还原酶 (GR)、谷胱甘肽过氧化物酶 (GPx)、谷胱甘肽 S-转移酶 (GSTs) 和胆碱酯酶 (ChEs) 的活性。结果表明，盐态铜和纳米态铜均导致白色盲颤蚓产生氧化应激和损伤，这可以通过 LPO 水平来证实，并且可以区分两种铜形态的作用。然而，尽管可以明显区分纳米态和盐态（时间和暴露时间依赖），但没有一种或一组生物标志物能够提供最佳的区分。
