College of Optometry, University of Houston, Houston, Texas 77204, USA.
Invest Ophthalmol Vis Sci. 2011 Jul 23;52(8):5473-80. doi: 10.1167/iovs.11-7347.
The ability to consistently resolve lamina cribrosa pores in vivo has applications in the study of optic nerve head and retinal disease mechanisms. Repeatability was assessed in imaging laminar pores in normal living eyes with a confocal adaptive optics scanning laser ophthalmoscope (AOSLO).
Reflectance images (840 nm) of the anterior lamina cribrosa were acquired using the AOSLO in four or more different sessions in two normal rhesus monkey eyes and three normal human eyes. Laminar pore areas, elongations (ratio of major to minor axes of the best-fit ellipse) and nearest neighbor distances were calculated for each session. Measurement repeatability was assessed across sessions.
Pore areas ranged from 90 to 4365 μm(2) in monkeys and 154 to 6637 μm(2) in humans. Mean variabilities in measuring pore area and elongation (i.e., mean of the standard deviation of measurements made across sessions for the same pores) were 50 μm(2) (6.1%) and 0.13 (6.7%), respectively, in monkeys and 113 μm(2) (8.3%) and 0.17 (7.7%), respectively, in humans. Mean variabilities in measuring nearest neighbor distances were 1.93 μm (5.2%) in monkeys and 2.79 μm (4.1%) in humans. There were no statistically significant differences in any pore parameters across sessions (ANOVA, P > 0.05).
The anterior lamina cribrosa was consistently imaged in vivo in normal monkey and human eyes. The small intersession variability in normal pore geometry suggests that AOSLO imaging could be used to measure and track changes in laminar pores in vivo during glaucomatous progression.
能够在活体中持续分辨出视盘筛板孔具有研究视神经头和视网膜疾病机制的应用价值。利用共焦自适应光学扫描激光检眼镜（AOSLO）在正常活体眼中成像，评估了在不同时间重复成像视盘筛板孔的能力。
在两只正常猕猴眼和三只正常人眼中，用 AOSLO 采集前筛板的反射图像（840nm），并在 4 个或更多不同的时间点采集。计算每个时间点的筛板孔面积、伸长率（最佳拟合椭圆的长轴与短轴之比）和最近邻距离。评估各时间点的测量重复性。
在猴子中，孔面积范围为 90 至 4365μm(2)，在人类中为 154 至 6637μm(2)。在猴子中，测量孔面积和伸长率的平均值变异（即同一孔在不同时间点测量的标准偏差的平均值）分别为 50μm(2)（6.1%）和 0.13（6.7%），在人类中分别为 113μm(2)（8.3%）和 0.17（7.7%）。测量最近邻距离的平均值变异在猴子中为 1.93μm（5.2%），在人类中为 2.79μm（4.1%）。在各时间点，各孔参数均无统计学差异（方差分析，P＞0.05）。
正常猕猴眼和人眼中的前筛板可在活体中持续成像。正常孔几何形状的时间点间变异性小，表明 AOSLO 成像可用于测量和跟踪青光眼进展过程中活体筛板孔的变化。
