Department of Forensic Medicine, Central District Office, National Forensic Service, Daejeon, 305-348, Korea.
Mol Cells. 2011 Jul;32(1):15-9. doi: 10.1007/s10059-011-2288-4. Epub 2011 May 16.
Genotyping of highly polymorphic short tandem repeat (STR) markers is widely used for the genetic identification of individuals in forensic DNA analyses and in paternity disputes. The National DNA Profile Databank recently established by the DNA Identification Act in Korea contains the computerized STR DNA profiles of individuals convicted of crimes. For the establishment of a large autosomal STR loci population database, 1805 samples were obtained at random from Korean individuals and 15 autosomal STR markers were analyzed using the AmpFlSTR Identifiler PCR Amplification kit. For the 15 autosomal STR markers, no deviations from the Hardy-Weinberg equilibrium were observed. The most informative locus in our data set was the D2S1338 with a discrimination power of 0.9699. The combined matching probability was 1.521 × 10(-17). This large STR profile dataset including atypical alleles will be important for the establishment of the Korean DNA database and for forensic applications.
基因分型高度多态性短串联重复（STR）标记广泛用于法医 DNA 分析和亲子关系纠纷中的个体遗传鉴定。韩国最近根据《DNA 鉴定法》建立了国家 DNA 特征数据库，其中包含了犯罪者的计算机化 STR DNA 特征。为了建立一个大型常染色体 STR 基因座群体数据库，从韩国个体中随机抽取了 1805 个样本，并使用 AmpFlSTR Identifiler PCR 扩增试剂盒分析了 15 个常染色体 STR 标记物。对于这 15 个常染色体 STR 标记物，没有观察到偏离哈迪-温伯格平衡。我们数据集中信息量最大的基因座是 D2S1338，其鉴别力为 0.9699。联合匹配概率为 1.521×10(-17)。这个包括非典型等位基因的大型 STR 图谱数据集对于建立韩国 DNA 数据库和法医学应用非常重要。
