高效液相色谱法测定珍菊降压片中绿原酸和氢氯噻嗪的含量
[HPLC determination of the contents chlorogenic acid and hydrochlorothiazide in zhenjujiangyapian].
作者信息
Gong Qinhong, Ruan Jian
机构信息
Yantai Yuhuangding Hospital, Yantai 264000, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Feb;36(4):481-3.
OBJECTIVE
To establish an HPLC method for determination of chlorogenic acid and hydrochlorothiazide in Zhenjujiangyapian.
METHOD
The HPLC method was carried on C18 column using methanol-0.1% phosphoric acid(20: 80) as mobile phase, and the detection wavelength was 327 nm, the flow rate was 1.0 mL x min(-1) and the temperature of column was 40 degrees C.
RESULT
In the HPLC method, the calibration curve for chlorogenic acid, hydrochlorothiazide were linear in the range of 0.049 6-0.496 (r = 0.999 5) and 1.002-10.02 microg (r = 0.999 8). The average recovery for chlorogenic acid, hydrochlorothiazide were 101.0% and 100. 1%. RSD were 2.0% and 1.4% (n = 9), respectively.
CONCLUSION
The method is convenient, precise and reliable for determining the content of chlorogenic acid and hydrochlorothiazide in Zhenjujiangyapian.
目的
建立高效液相色谱法测定珍菊降压片中绿原酸和氢氯噻嗪的含量。
方法
采用高效液相色谱法，以十八烷基硅烷键合硅胶为填充剂；以甲醇 - 0.1%磷酸溶液（20∶80）为流动相；检测波长为327nm；流速为1.0ml·min-1；柱温为40℃。
结果
绿原酸在0.049 6～0.496μg范围内线性关系良好（r = 0.999 5），氢氯噻嗪在1.002～10.02μg范围内线性关系良好（r = 0.999 8）。绿原酸平均回收率为101.0%，氢氯噻嗪平均回收率为100.1%，相对标准偏差分别为2.0%和1.4%（n = 9）。
结论
该方法简便、准确、可靠，可用于珍菊降压片中绿原酸和氢氯噻嗪的含量测定。
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