Altanerova V, Ban J, Kettmann R, Altaner C
Department of Molecular Virology, Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Arch Geschwulstforsch. 1990;60(2):89-96.
Bovine leukemia virus (BLV) was inoculated into one-day-old chickens. In a small part of inoculated chickens leukemia developed during observation period of one year. Out of 88 birds inoculated, only 4 developed histopathologically verified leukemia. The induced leukemia was characterized by enlarged liver and spleen. The organs were infiltrated with leukemic cells. The DNAs of body organs of inoculated chickens were analysed by Southern blot hybridization for the presence of BLV specific sequences. Out of 9 suspicious chickens tested in 6 birds the BLV was found to be integrated into host DNA either as a complete viral genome or as a part corresponding to its 3'-end. The leukemic cells were monoclonal as regard to the integration site of the BLV provirus. Neither the expression of BLV provirus in chicken leukemic cells nor the antibody response to BLV antigens in inoculated birds was detected. The rearrangements and amplification of erb-B and myb loci of protooncogenes in leukemic cells was detected. There were no changes in loci of following protooncogenes: myc, sis, fes, fps, erb A, src and yes. All obtained data taken together suggest that the BLV induced leukemia in chickens is caused by insertional mutagenesis.
将牛白血病病毒（BLV）接种到1日龄的鸡体内。在一年的观察期内，一小部分接种的鸡发生了白血病。在88只接种的鸡中，只有4只经组织病理学证实发生了白血病。诱发的白血病的特征是肝脏和脾脏肿大。这些器官被白血病细胞浸润。通过Southern印迹杂交分析接种鸡身体器官的DNA，以检测是否存在BLV特异性序列。在检测的9只可疑鸡中，6只鸡的BLV被发现以完整病毒基因组或其3'端对应部分的形式整合到宿主DNA中。就BLV前病毒的整合位点而言，白血病细胞是单克隆的。在鸡白血病细胞中未检测到BLV前病毒的表达，在接种的鸡中也未检测到对BLV抗原的抗体反应。在白血病细胞中检测到原癌基因erb-B和myb位点的重排和扩增。以下原癌基因的位点没有变化：myc、sis、fes、fps、erb A、src和yes。综合所有获得的数据表明，BLV诱发的鸡白血病是由插入诱变引起的。
