Department of Nanomedicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Int J Nanomedicine. 2011;6:915-28. doi: 10.2147/IJN.S19013. Epub 2011 Apr 28.
The process of laser-mediated ablation of cancer cells marked with biofunctionalized carbon nanotubes is frequently called "nanophotothermolysis". We herein present a method of selective nanophotothermolisys of pancreatic cancer (PC) using multiwalled carbon nanotubes (MWCNTs) functionalized with human serum albumin (HSA). With the purpose of testing the therapeutic value of these nanobioconjugates, we have developed an ex-vivo experimental platform. Surgically resected specimens from patients with PC were preserved in a cold medium and kept alive via intra-arterial perfusion. Additionally, the HSA-MWCNTs have been intra-arterially administered in the greater pancreatic artery under ultrasound guidance. Confocal and transmission electron microscopy combined with immunohistochemical staining have confirmed the selective accumulation of HSA-MWCNTs inside the human PC tissue. The external laser irradiation of the specimen has significantly produced extensive necrosis of the malign tissue after the intra-arterial administration of HSA-MWCNTs, without any harmful effects on the surrounding healthy parenchyma. We have obtained a selective photothermal ablation of the malign tissue based on the selective internalization of MWCNTs with HSA cargo inside the pancreatic adenocarcinoma after the ex-vivo intra-arterial perfusion.
用生物功能化碳纳米管标记的癌细胞的激光介导消融过程通常被称为“光热解”。我们在此介绍了一种使用人血清白蛋白（HSA）功能化的多壁碳纳米管（MWCNT）选择性光热解胰腺癌细胞（PC）的方法。为了测试这些纳米生物缀合物的治疗价值，我们开发了一种离体实验平台。从 PC 患者手术切除的标本在冷介质中保存，并通过动脉内灌注保持存活。此外，在超声引导下，HSA-MWCNTs 经胰大动脉内给药。共聚焦和透射电子显微镜结合免疫组织化学染色证实了 HSA-MWCNTs 在人 PC 组织内的选择性积累。在 HSA-MWCNTs 动脉内给药后，对标本进行外部激光照射，可显著产生恶性组织的广泛坏死，而对周围健康的实质组织没有任何有害影响。我们已经获得了基于 MWCNT 与 HSA 货物在胰腺腺癌中的选择性内化的离体动脉内灌注后的恶性组织的选择性光热消融。
