不同结构果胶片段的微生物利用和选择性。
Microbial utilization and selectivity of pectin fractions with various structures.
机构信息
Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading, United Kingdom.
出版信息
Appl Environ Microbiol. 2011 Aug 15;77(16):5747-54. doi: 10.1128/AEM.00179-11. Epub 2011 Jul 1.
Abstract
To evaluate the fermentation properties of oligosaccharides derived from pectins and their parent polysaccharides, a 5-ml-working-volume, pH- and temperature-controlled fermentor was tested. Six pectic oligosaccharides representing specific substructures found within pectins were prepared. These consisted of oligogalacturonides (average degrees of polymerization [DP] of 5 and 9), methylated oligogalacturonides (average DP of 5), oligorhamnogalacturonides (average DP of 10 as a disaccharide unit of galacturonic acid and rhamnose), oligogalactosides (average DP of 5), and oligoarabinosides (average DP of 6). The influence of these carbohydrates on the human fecal microbiota was evaluated. Use of neutral sugar fractions resulted in an increase in Bifidobacterium populations and gave higher organic acid yields. The Bacteroides-Prevotella group significantly increased on all oligosaccharides except oligogalacturonides with an average DP of 5. The most selective substrates for bifidobacteria were arabinan, galactan, oligoarabinosides, and oligogalactosides.
摘要
为了评估果胶及其母体多糖衍生的低聚糖的发酵特性，我们测试了一个 5 毫升工作体积、pH 值和温度可控的发酵罐。我们制备了 6 种果胶低聚糖，它们代表了果胶中存在的特定亚结构。这些低聚糖包括寡半乳糖醛酸（聚合度[DP]平均为 5 和 9）、甲基化寡半乳糖醛酸（DP 平均为 5）、寡鼠李半乳糖醛酸（作为半乳糖醛酸和鼠李糖的二糖单位，DP 平均为 10）、寡半乳糖苷（DP 平均为 5）和寡阿拉伯糖苷（DP 平均为 6）。我们评估了这些碳水化合物对人类粪便微生物群的影响。使用中性糖馏分导致双歧杆菌种群增加，并产生更高的有机酸产量。除 DP 平均为 5 的寡半乳糖醛酸外，拟杆菌-普雷沃氏菌组在所有低聚糖上均显著增加。双歧杆菌最具选择性的底物是阿拉伯聚糖、半乳聚糖、寡阿拉伯糖苷和寡半乳糖苷。
相似文献
1
Microbial utilization and selectivity of pectin fractions with various structures.不同结构果胶片段的微生物利用和选择性。
Appl Environ Microbiol. 2011 Aug 15;77(16):5747-54. doi: 10.1128/AEM.00179-11. Epub 2011 Jul 1.
2
In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora.人肠道微生物群对甜菜阿拉伯聚糖和阿拉伯低聚糖的体外发酵
J Appl Microbiol. 2006 Feb;100(2):407-14. doi: 10.1111/j.1365-2672.2005.02780.x.
3
Comparison of the in vitro bifidogenic properties of pectins and pectic-oligosaccharides.果胶和果胶寡糖的体外双歧杆菌增殖特性比较。
J Appl Microbiol. 2002;93(3):505-11. doi: 10.1046/j.1365-2672.2002.01719.x.
4
Purification, characterization, and prebiotic properties of pectic oligosaccharides from orange peel wastes.橙皮废料中果胶寡糖的纯化、表征及益生元特性
J Agric Food Chem. 2014 Oct 8;62(40):9769-82. doi: 10.1021/jf503475b. Epub 2014 Sep 23.
5
In vitro fermentation properties of pectins and enzymatic-modified pectins obtained from different renewable bioresources.不同可再生生物资源来源的果胶及其酶修饰果胶的体外发酵特性。
Carbohydr Polym. 2018 Nov 1;199:482-491. doi: 10.1016/j.carbpol.2018.07.041. Epub 2018 Jul 21.
6
Feruloylated and nonferuloylated arabino-oligosaccharides from sugar beet pectin selectively stimulate the growth of Bifidobacterium spp. in human fecal in vitro fermentations.糖甜菜果胶中的阿魏酰化和非阿魏酰化阿拉伯低聚糖在人体粪便体外发酵中选择性地刺激双歧杆菌属的生长。
J Agric Food Chem. 2011 Jun 22;59(12):6511-9. doi: 10.1021/jf200996h. Epub 2011 May 25.
7
Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation.通过体外实验评估新型低聚葡聚糖作为益生元用于肥胖管理的潜力。
Br J Nutr. 2014 Oct 28;112(8):1303-14. doi: 10.1017/S0007114514002177. Epub 2014 Sep 8.
8
In vitro fermentation of lactulose-derived oligosaccharides by mixed fecal microbiota.混合粪便微生物群体外发酵乳果糖衍生低聚糖。
J Agric Food Chem. 2012 Feb 29;60(8):2024-32. doi: 10.1021/jf203622d. Epub 2012 Feb 15.
9
Degradation of pectins with different degrees of esterification by Bacteroides thetaiotaomicron isolated from human gut flora.从人类肠道菌群中分离出的多形拟杆菌对不同酯化度果胶的降解作用。
Appl Environ Microbiol. 2000 Apr;66(4):1321-7. doi: 10.1128/AEM.66.4.1321-1327.2000.
10
In vitro fermentation of linear and alpha-1,2-branched dextrans by the human fecal microbiota.人粪便微生物对线性和α-1,2-分支葡聚糖的体外发酵。
Appl Environ Microbiol. 2011 Aug;77(15):5307-15. doi: 10.1128/AEM.02568-10. Epub 2011 Jun 10.
引用本文的文献
1
Exo- and Endo-1,5-α-L-Arabinanases and Prebiotic Arabino-Oligosaccharides Production.外切和内切1,5-α-L-阿拉伯聚糖酶与益生元阿拉伯低聚糖的生产
J Microbiol Biotechnol. 2025 Jan 13;35:e2412052. doi: 10.4014/jmb.2412.12052.
2
Production of Pectic Oligosaccharides from Citrus Peel via Steam Explosion.通过蒸汽爆破从柑橘皮中制备果胶寡糖
Foods. 2024 Nov 22;13(23):3738. doi: 10.3390/foods13233738.
3
Dietary fibre and the gut-brain axis: microbiota-dependent and independent mechanisms of action.膳食纤维与肠-脑轴：微生物群依赖和独立的作用机制。
Gut Microbiome (Camb). 2021 Sep 8;2:e3. doi: 10.1017/gmb.2021.3. eCollection 2021.
4
Effects of Purified Vitexin and Iso-Vitexin from Mung Bean Seed Coat on Antihyperglycemic Activity and Gut Microbiota in Overweight Individuals' Modulation.纯化的豇豆花苷和异豇豆花苷对超重人群降糖活性和肠道微生物群的调节作用。
Nutrients. 2024 Sep 6;16(17):3017. doi: 10.3390/nu16173017.
5
Simulated Swine Digestion and Gut Microbiota Fermentation of Hydrolyzed Copra Meal.水解椰肉粕的模拟猪消化及肠道微生物群发酵
Animals (Basel). 2024 Jun 4;14(11):1677. doi: 10.3390/ani14111677.
6
A Gestational Pectin Diet Could Improve the Health of Multiparous Sows by Modulating the Gut Microbiota and Cytokine Level during Late Pregnancy.妊娠期果胶饮食可通过调节妊娠后期经产母猪的肠道微生物群和细胞因子水平来改善其健康状况。
Animals (Basel). 2024 May 24;14(11):1559. doi: 10.3390/ani14111559.
7
The Utilization by spp. of a Purified Polysaccharide from Fuzhuan Brick Tea.某些物种对茯砖茶中纯化多糖的利用情况
Foods. 2024 May 26;13(11):1666. doi: 10.3390/foods13111666.
8
Structural characterization of strawberry pomace.草莓渣的结构表征
Heliyon. 2024 Apr 17;10(9):e29787. doi: 10.1016/j.heliyon.2024.e29787. eCollection 2024 May 15.
9
Enhancing enzymatic production efficiency of crude pectic oligosaccharides by pulsed electric field and study of prebiotic potential.脉冲电场提高粗制果胶寡糖的酶促生产效率及益生元潜力研究
J Food Sci Technol. 2024 Feb;61(2):320-330. doi: 10.1007/s13197-023-05843-8. Epub 2023 Sep 29.
10
Age-related effects on the modulation of gut microbiota by pectins and their derivatives: an study.果胶及其衍生物对肠道微生物群调节的年龄相关影响：一项研究。
Front Microbiol. 2023 Jul 5;14:1207837. doi: 10.3389/fmicb.2023.1207837. eCollection 2023.
本文引用的文献
1
Monoclonal antibodies to rhamnogalacturonan I backbone.针对鼠李半乳糖醛酸聚糖 I 骨架的单克隆抗体。
Planta. 2010 May;231(6):1373-83. doi: 10.1007/s00425-010-1116-y. Epub 2010 Mar 23.
2
Probiotic and gut lactobacilli and bifidobacteria: molecular approaches to study diversity and activity.益生菌与肠道乳酸杆菌和双歧杆菌：研究多样性与活性的分子方法
Annu Rev Microbiol. 2009;63:269-90. doi: 10.1146/annurev.micro.091208.073341.
3
Modulation of the fecal microflora profile and immune function by a novel trans-galactooligosaccharide mixture (B-GOS) in healthy elderly volunteers.新型低聚半乳糖混合物（B-GOS）对健康老年志愿者粪便微生物群谱和免疫功能的调节作用
Am J Clin Nutr. 2008 Nov;88(5):1438-46. doi: 10.3945/ajcn.2008.26242.
4
Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients.普拉梭菌是一种通过对克罗恩病患者肠道微生物群分析鉴定出的抗炎共生细菌。
Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16731-6. doi: 10.1073/pnas.0804812105. Epub 2008 Oct 20.
5
From bacterial genome to functionality; case bifidobacteria.从细菌基因组到功能；以双歧杆菌为例
Int J Food Microbiol. 2007 Nov 30;120(1-2):2-12. doi: 10.1016/j.ijfoodmicro.2007.06.011. Epub 2007 Jun 13.
6
Pectin induces apoptosis in human prostate cancer cells: correlation of apoptotic function with pectin structure.果胶诱导人前列腺癌细胞凋亡：凋亡功能与果胶结构的相关性。
Glycobiology. 2007 Aug;17(8):805-19. doi: 10.1093/glycob/cwm054. Epub 2007 May 19.
7
Prebiotics: the concept revisited.益生元：概念再探讨。
J Nutr. 2007 Mar;137(3 Suppl 2):830S-7S. doi: 10.1093/jn/137.3.830S.
8
In vitro evaluation of the prebiotic activity of a pectic oligosaccharide-rich extract enzymatically derived from bergamot peel.对一种酶法从佛手柑果皮中提取的富含果胶寡糖提取物的益生元活性进行体外评估。
Appl Microbiol Biotechnol. 2007 Jan;73(5):1173-9. doi: 10.1007/s00253-006-0561-9. Epub 2006 Oct 5.
9
In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora.人肠道微生物群对甜菜阿拉伯聚糖和阿拉伯低聚糖的体外发酵
J Appl Microbiol. 2006 Feb;100(2):407-14. doi: 10.1111/j.1365-2672.2005.02780.x.
10
In vitro determination of prebiotic properties of oligosaccharides derived from an orange juice manufacturing by-product stream.体外测定源自橙汁生产副产物流的低聚糖的益生元特性。
Appl Environ Microbiol. 2005 Dec;71(12):8383-9. doi: 10.1128/AEM.71.12.8383-8389.2005.
Zotero 插件