Laboratory of Animal Nutrition and Animal Product Quality, Department of Animal Production, Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, 9090, Melle, Belgium.
Plant Foods Hum Nutr. 2011 Jun;66(2):196-202. doi: 10.1007/s11130-011-0233-5.
The absorption, distribution and elimination of carnosic acid, the main antioxidant found in rosemary was studied, in vivo, in rats. Therefore, carnosic acid was administrated in a single dose, intravenously (20.5 ± 4.2 mg/kg) and orally (64.3 ± 5.8 mg/kg), to four and nine rats, respectively. Blood samples were collected at different time points, and plasma concentrations of carnosic acid were determined using LC-MS. Furthermore, total collection of urine and feces was done during 4 h and 24 h for the intravenous and oral administrations, respectively. After euthanizing the rats, intestinal content, liver and muscle tissue were sampled to determine carnosic acid concentrations. The bioavailability of carnosic acid, after 360 min, was 40.1%. Traces of carnosic acid were found in the rats intestinal content, liver and muscle tissue of abdomen and legs. The recovery of carnosic acid in the feces, 24 h after oral administration, was 15.6 ± 8.2%. Carnosic acid is absorbed into the bloodstream after oral administration in rats and is therefore bioavailable. It was found that carnosic acid in vivo is present in its free form and that its main elimination route is the fecal route.
本研究旨在体内研究迷迭香中主要抗氧化剂迷迭香酸的吸收、分布和消除。为此，以单剂量形式分别经静脉（20.5 ± 4.2 mg/kg）和口服（64.3 ± 5.8 mg/kg）给予 4 只和 9 只大鼠迷迭香酸。分别在不同时间点采集血样，并采用 LC-MS 测定血浆中迷迭香酸的浓度。此外，还分别在静脉和口服给药后 4 小时和 24 小时内收集 4 小时和 24 小时的尿液和粪便。安乐死后，采集大鼠肠道内容物、肝脏和腹部及腿部肌肉组织以测定迷迭香酸浓度。360 分钟后，迷迭香酸的生物利用度为 40.1%。在大鼠的肠道内容物、肝脏和腹部及腿部肌肉组织中均发现有迷迭香酸的痕迹。口服给药 24 小时后，粪便中迷迭香酸的回收率为 15.6 ± 8.2%。口服给药后，迷迭香酸被吸收进入大鼠的血液，因此具有生物利用度。研究发现，体内迷迭香酸以游离形式存在，其主要消除途径是粪便途径。
