在高度多样化的环境中， spoligotyping 和可变数目串联重复序列分型对牛分枝杆菌分子溯源的局限性。
Limitations of spoligotyping and variable-number tandem-repeat typing for molecular tracing of Mycobacterium bovis in a high-diversity setting.
机构信息
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain.
出版信息
J Clin Microbiol. 2011 Sep;49(9):3361-4. doi: 10.1128/JCM.00301-11. Epub 2011 Jul 13.
Abstract
This study describes the attempt to trace the first Mycobacterium bovis outbreak in alpacas (Lama pacos) in Spain by spoligotyping and variable-number tandem-repeat (VNTR) analysis. Due to high genotype diversity, no matching source was identified, but local expansion of a clonal group was found and its significance for molecular tracing is discussed.
摘要
本研究通过 spoligotyping 和可变数目串联重复（VNTR）分析，尝试追踪西班牙驼羊（Lama pacos）中的首例牛分枝杆菌爆发。由于基因型多样性较高，未发现匹配的来源，但发现了一个克隆群的本地扩张，并讨论了其对分子追踪的意义。
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