Jahn G, Harthus H P, Bröker M, Borisch B, Platzer B, Plachter B
Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg.
Klin Wochenschr. 1990 Oct 17;68(20):1003-7. doi: 10.1007/BF01646545.
Procedures for diagnostics of cytomegalovirus infections include histopathology, cell culture, serology, and direct detection of viral antigens or nucleic acids within infected cells or tissues. In order to develop a new diagnostic reagent for viral antigen detection, we generated a mouse monoclonal antibody. This antibody was raised against a recombinant antigen representing part of the large phosphorylated structural protein pp150 of human cytomegalovirus. The monoclonal antibody was shown to be useful for antigen detection by immunofluorescence and immunoenzymatic staining in infected cells from cell culture as well as from infected organs. The antibody proved to be reactive even in paraffin-embedded sections from tissue specimens.
巨细胞病毒感染的诊断方法包括组织病理学、细胞培养、血清学以及直接检测受感染细胞或组织中的病毒抗原或核酸。为了开发一种用于病毒抗原检测的新型诊断试剂，我们制备了一种小鼠单克隆抗体。该抗体是针对一种重组抗原产生的，该重组抗原代表人类巨细胞病毒大的磷酸化结构蛋白pp150的一部分。在来自细胞培养物以及受感染器官的受感染细胞中，通过免疫荧光和免疫酶染色显示该单克隆抗体可用于抗原检测。该抗体在组织标本的石蜡包埋切片中也显示有反应性。
