Molecular and Cellular Glycobiology Unit, Department of Biological Science, Sungkyunkwan University, 300 Chunchun-Dong, Jangan-Gu, Suwon City, Kyunggi 440-746, Republic of Korea; Department of Acupuncture, College of Oriental Medicine, Dongguk University, Kyungju 780-714, Republic of Korea.
Environ Toxicol Pharmacol. 2007 Mar;23(2):154-61. doi: 10.1016/j.etap.2006.08.002. Epub 2006 Aug 15.
Ulmus davidiana Planch (Ulmaceae) (UD) frequently appears as the main ingredient in prescriptions for bone injuries, however, the action mechanism is unclear. In the present study, (i) the effect of the aqueous extract of UD on bone cells was investigated in vitro and (ii) the immunomodulatory activity of UD was investigated with regard to cellular and humoral immunity. The osteoprecursor cells (OPC) were incubated in the medium with different concentrations of the UD and the cell proliferation was studied. When the concentration of UD was <100μg/ml, the proliferation of OPC was enhanced. However, the proliferation of OPC was inhibited by UD with the concentrations >180μg/ml. Under most treatments, the cells presented low expression for cyclooxygenase-2 (Cox-2) protein. On the other hand, oral administration of the ethanolic and water extracts of UD, at the doses of 20, 50, 100 and 200mg/kg in mice, dose-dependently potentiated the delayed-type hypersensitivity reaction induced both by sheep red blood cells (SRBC) and oxazolone. It significantly enhanced the production of circulating antibody titers in mice in response to SRBC. UD had no any effect on macrophage phagocytosis. Chronic administration of UD significantly ameliorated the total white blood cell counts and also restored the myelosuppressive effects induced by cyclophosphamide. From the results, it was concluded that UD directly stimulates the proliferation, alkaline phosphatase activity, protein secretion and particularly type I collagen synthesis of OPC in a dose-dependent manner, and that UD possesses immunomodulatory activity.
梨树（榆科）（UD）常作为骨伤处方的主要成分出现，但作用机制尚不清楚。本研究（i）在体外研究了 UD 水提取物对骨细胞的作用，（ii）研究了 UD 对细胞和体液免疫的免疫调节活性。将成骨前体细胞（OPC）在含有不同浓度 UD 的培养基中孵育，并研究细胞增殖情况。当 UD 浓度<100μg/ml 时，OPC 增殖增强。然而，当 UD 浓度>180μg/ml 时，OPC 的增殖受到抑制。在大多数处理下，细胞 COX-2（环氧化酶-2）蛋白表达水平较低。另一方面，在小鼠中口服 UD 的乙醇和水提取物，剂量为 20、50、100 和 200mg/kg，可剂量依赖性增强绵羊红细胞（SRBC）和 2,4-二硝基氟苯诱导的迟发型超敏反应。它显著增强了小鼠对 SRBC 的循环抗体滴度的产生。UD 对巨噬细胞吞噬作用没有任何影响。UD 的慢性给药显著改善了总白细胞计数，并恢复了环磷酰胺诱导的骨髓抑制作用。从结果中可以得出结论，UD 以剂量依赖的方式直接刺激 OPC 的增殖、碱性磷酸酶活性、蛋白质分泌，特别是 I 型胶原合成，并且 UD 具有免疫调节活性。
