Molecular and Cellular Glycobiology Unit, Department of Biological Science, Sungkyunkwan University, Chunchun-Dong 300, Suwon City, Kyunggi-Do 440-746, Republic of Korea; Department of Oriental Medicine, Dongguk University, Kyungju, Kyungbuk 780-714, Republic of Korea.
Environ Toxicol Pharmacol. 2007 Jan;23(1):102-10. doi: 10.1016/j.etap.2006.07.013. Epub 2006 Aug 12.
Ulmus davidiana Planch (Ulmaceae) extract (UD) has long been known to have anti-inflammatory and anticancer activities. UD has been also known to have protective effects on damaged tissue, inflammation and bone among other functions. Effects of UD on inflammatory and immune responses and its mechanisms in collagen-induced inflammation (CII) rat were studied. Hind paw volumes of rats were measured by volume meter; lymphocyte proliferation, interleukin (IL)-1, IL-2, tumor necrosis factor (TNF)-α level was determined by 3-(4,5-2dimethylthiazal-2yl)2,5-diphenyltetrazoliumbromide assay. Antibodies to collagen type II (BC-II) were determined by enzyme-linked immunosorbent assay. There was a marked secondary inflammatory response in CII model, which accompanied with the decrease of body weight and the weight of immune organs simultaneously. The administration of UD (20, 80, 150mg/kg, intragastrically×10 days) inhibited the inflammatory response and restored body weight and the weight of immune organs of CII rats. Lymphocyte proliferation and IL-2 production of CII rats increases, together with IL-1 and TNF-α in peritoneal macrophages and synoviocytes. The administration of UD (20, 80, 150mg/kg, 10 days) reduced above changes significantly. UD had no effect on the concentration of antibodies to BC-II. From the results, it was concluded that UD possesses anti-inflammatory and immunoregulatory activities and has a therapeutic effect on CII rats.
榆树（榆科）提取物（UD）长期以来一直被认为具有抗炎和抗癌活性。UD 还具有保护受损组织、炎症和骨骼等功能。本研究旨在探讨 UD 对胶原诱导性炎症（CII）大鼠炎症和免疫反应的影响及其机制。通过体积计测量大鼠后爪体积；通过 3-（4,5-2-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐测定淋巴细胞增殖、白细胞介素（IL）-1、IL-2、肿瘤坏死因子（TNF）-α水平。通过酶联免疫吸附试验测定抗 II 型胶原抗体（BC-II）。CII 模型中存在明显的二次炎症反应，同时伴随着体重和免疫器官重量的减轻。UD（20、80、150mg/kg，灌胃×10 天）给药抑制了 CII 大鼠的炎症反应，恢复了其体重和免疫器官重量。CII 大鼠的淋巴细胞增殖和 IL-2 产生增加，同时腹腔巨噬细胞和滑膜细胞中的 IL-1 和 TNF-α也增加。UD（20、80、150mg/kg，10 天）给药显著降低了上述变化。UD 对 BC-II 抗体的浓度没有影响。结果表明，UD 具有抗炎和免疫调节活性，对 CII 大鼠具有治疗作用。
