Fisheries College, Ocean University of China, Qingdao, 266003, China.
Mol Biol Rep. 2012 Apr;39(4):4249-59. doi: 10.1007/s11033-011-1211-6. Epub 2011 Jul 24.
The complete mitochondrial genome of the spinyhead croaker Collichthys lucidus was determined using long-PCR and primer walking methods. It is a circular molecule of 16,451 bp in length with a standard set of 22 tRNAs, 2 rRNAs, 13 protein-coding genes as well as a non-coding control region in the same order as those of the other bony fishes. C. lucidus mitogenome exhibited a clear strand-specific bias in nucleotide composition, as evidence by a GC- skew of the H-strand of -0.319. The strand-specific bias was also reflected in the codon usage of genes oriented in opposite directions. All tRNA genes except for tRNA( Ser(AGY)) harbored the typical cloverleaf secondary structures and possessed anticodons that matched the vertebrate mitochondrial genetic code. Contrary to the typical structure of control region consistig of TAS, central, and CSB domains, there were no central conserved blocks available in C. lucidus mitogenome. Despite extensive studies based on both morphology and molecules, phylogenetic position of C. lucidus with Sciaenidae is still controversial. Our phylogenetic results provided more evidence to support previous morphological studies and consistently placed C. lucidus as a sister taxon to Collichthys niveatus, with both of these taxa forming the monophyletic Collichthys.
利用长 PCR 和引物步行方法测定了尖嘴鱚（Collichthys lucidus）的完整线粒体基因组。它是一个 16451bp 的圆形分子，具有标准的 22 个 tRNA、2 个 rRNA、13 个蛋白编码基因以及与其他硬骨鱼类相同顺序的非编码控制区。C. lucidus 线粒体基因组在核苷酸组成上表现出明显的链特异性偏倚，这一点可以通过 H 链的 GC 倾斜度-0.319 来证明。这种链特异性偏倚也反映在相反方向取向的基因的密码子使用上。除了 tRNA(Ser(AGY))之外，所有的 tRNA 基因都具有典型的三叶草二级结构，并具有与脊椎动物线粒体遗传密码相匹配的反密码子。与由 TAS、中央和 CSB 结构域组成的典型控制区结构相反，C. lucidus 线粒体基因组中没有中央保守区。尽管基于形态和分子进行了广泛的研究，但 C. lucidus 与石首鱼科的系统发育位置仍然存在争议。我们的系统发育结果提供了更多的证据支持先前的形态学研究，并一致将 C. lucidus 置于尖嘴鱚（Collichthys niveatus）的姊妹分类群中，这两个分类群形成了单系的 Collichthys 。
