Lemieux C, St-Germain G, Vincelette J, Kaufman L, de Repentigny L
Notre-Dame, Hospital, Montreal, Quebec, Canada.
J Clin Microbiol. 1990 Feb;28(2):249-53. doi: 10.1128/jcm.28.2.249-253.1990.
The Cand-Tec Candida detection system and enzyme immunoassay for serum mannan were retrospectively compared in a controlled collaborative evaluation of antigen detection in 32 patients with candidiasis proven by biopsy or culture from a normally sterile site and with sera drawn within 7 days of inclusion. With a threshold titer of 1/8, which excluded false-positive results in 17 hospitalized patients without candidiasis, sensitivities for all 32 patients with candidiasis were 44% for the Cand-Tec assay and 17% for the enzyme immunoassay. Both assays provided greater sensitivity when sera were drawn within 24 h of inclusion in the study and in the category of patients with invasive candidiasis (57% by Cand-Tec and 33% by enzyme immunoassay). The Cand-Tec assay gave false-positive results (titer, greater than or equal to 1/8) in 4 of 6 patients with transient candidemia, in 1 of 20 otherwise healthy patients with rheumatoid factor, and in 1 patient with a positive cryptococcal latex agglutination test. Three serum specimens from 3 of 32 patients with candidiasis contained rheumatoid factor and gave titers of greater than or equal to 1/8 by the Cand-Tec assay. Detection of serum mannan by enzyme immunoassay was less sensitive but more specific than the Cand-Tec Candida detection system for the diagnosis of invasive candidiasis.
在一项对照协作评估中，对32例经活检或从正常无菌部位培养证实患有念珠菌病且在纳入研究后7天内采集血清的患者，回顾性比较了Cand-Tec念珠菌检测系统和血清甘露聚糖酶免疫测定法检测抗原的情况。设定阈值滴度为1/8，该阈值可排除17例无念珠菌病的住院患者的假阳性结果，对于所有32例念珠菌病患者，Cand-Tec检测法的敏感性为44%，酶免疫测定法的敏感性为17%。当在纳入研究后24小时内采集血清时，以及在侵袭性念珠菌病患者类别中，两种检测方法的敏感性均更高（Cand-Tec检测法为57%，酶免疫测定法为33%）。Cand-Tec检测法在6例短暂念珠菌血症患者中的4例、20例其他方面健康但患有类风湿因子的患者中的1例以及1例隐球菌乳胶凝集试验阳性的患者中出现了假阳性结果（滴度大于或等于1/8）。32例念珠菌病患者中有3例的三份血清标本含有类风湿因子，经Cand-Tec检测法检测，其滴度大于或等于1/8。对于侵袭性念珠菌病的诊断，酶免疫测定法检测血清甘露聚糖的敏感性较低，但比Cand-Tec念珠菌检测系统更具特异性。
