Oliveira Manuel, Bosco Nabil, Perruisseau Genevieve, Nicolas Jeanne, Segura-Roggero Iris, Duboux Stéphane, Briand Muriel, Blum Stéphanie, Benyacoub Jalil
Nutrition and Health Department, Nestlé Research Center, CH 1000 Lausanne, Switzerland.
Clin Dev Immunol. 2011;2011:807483. doi: 10.1155/2011/807483. Epub 2011 Jul 25.
Studies showed that specific probiotics provide therapeutic benefits in inflammatory bowel disease. In vitro evidence suggested that Lactobacillus paracasei also called ST11 (CNCM I-2116) is a potent strain with immune modulation properties. However, little is known about its capacity to alleviate inflammatory symptoms in vivo In this context, the main objective of this study was to investigate the role of ST11 on intestinal inflammation using the adoptive transfer mouse model of experimental colitis. Rag2(-/-) recipient mice were fed with ST11 (10(9) CFU/day)a month prior toinduce colitis by adoptive transfer of naive T cells. One month later, in clear contrast to nonfed mice, weight loss was significantly reduced by 50% in ST11-fed mice. Further analysis of colon specimens revealed a significant reduction neutrophil infiltration and mucosal expression of IL1β, IL-6, and IL12 proinflammatory cytokines, whereas no consistent differences in expression of antibacterial peptides or tight junction proteins were observed between PBS and ST11-fed mice. All together, our results demonstrate that oral administration of ST11 was safe and had a significant preventive effect on colitis. We conclude that probiotics such as Lactobacillus paracasei harbor worthwhile in vivo immunomodulatory properties to prevent intestinal inflammation by nutritional approaches.
研究表明，特定的益生菌在炎症性肠病中具有治疗作用。体外证据表明，副干酪乳杆菌（也称为ST11，保藏编号为CNCM I-2116）是一种具有免疫调节特性的有效菌株。然而，关于其在体内减轻炎症症状的能力知之甚少。在此背景下，本研究的主要目的是利用实验性结肠炎的过继转移小鼠模型来研究ST11在肠道炎症中的作用。在通过过继转移未致敏T细胞诱导结肠炎前一个月，给Rag2(-/-)受体小鼠喂食ST11（10⁹CFU/天）。一个月后，与未喂食的小鼠形成鲜明对比的是，喂食ST11的小鼠体重减轻显著减少了50%。对结肠标本的进一步分析显示，中性粒细胞浸润以及促炎细胞因子IL-1β、IL-6和IL-12的黏膜表达显著减少，而在PBS组和喂食ST11的小鼠之间，抗菌肽或紧密连接蛋白的表达未观察到一致的差异。总之，我们的结果表明，口服ST11是安全的，对结肠炎具有显著的预防作用。我们得出结论，像副干酪乳杆菌这样的益生菌具有值得关注的体内免疫调节特性，可通过营养方法预防肠道炎症。
