从卷枝毛霉中分离和鉴定阿非迪可林和厚垣孢子醇衍生物。

Isolation and characterization of aphidicolin and chlamydosporol derivatives from Tolypocladium inflatum.

机构信息

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.

出版信息

J Nat Prod. 2011 Aug 26;74(8):1798-804. doi: 10.1021/np200431k. Epub 2011 Aug 3.

DOI:10.1021/np200431k

PMID:21812410

Abstract

Six new secondary metabolites including two aphidicolin analogues, inflatins A (1) and B (2), and four chlamydosporol derivatives, inflatins C-F (3-6), have been isolated from the crude extract of Tolypocladium inflatum. The structures of 1-6 were determined mainly by NMR experiments, and 4 and 5 were further confirmed by X-ray crystallography. The absolute configurations of C-16 in 1 and C-5 in 3 were deduced via the circular dichroism data of the in situ formed [Rh₂(OCOCF₃)₄] complexes, whereas that of 4 was assigned by X-ray crystallography using Cu Kα radiation. Compounds 1 and 2 showed modest cytotoxicity against a panel of eight human tumor cell lines.

摘要

从土曲霉（Tolypocladium inflatum）的粗提物中分离得到了 6 种新的次生代谢产物，包括 2 种阿皮考林类似物、inflatin A（1）和 B（2）以及 4 种 chlamydosporol 衍生物 inflatins C-F（3-6）。通过 NMR 实验主要确定了 1-6 的结构，通过 X 射线晶体学进一步证实了 4 和 5 的结构。通过现场形成的[Rh₂（OCOCF₃）₄]配合物的圆二色性数据推断出 1 中 C-16 和 3 中 C-5 的绝对构型，而 4 的构型则通过使用 Cu Kα 辐射的 X 射线晶体学确定。化合物 1 和 2 对一组 8 个人类肿瘤细胞系表现出中等的细胞毒性。