Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA.
J Clin Microbiol. 2011 Nov;49(11):3849-54. doi: 10.1128/JCM.00619-11. Epub 2011 Sep 14.
Acinetobacter baumannii is emerging as an important nosocomial pathogen worldwide. We report molecular epidemiology of 65 carbapenem-nonsusceptible A. baumannii isolates identified from hospitals in New York, Pennsylvania, Florida, Missouri, Nevada, and California between 2008 and 2009. All isolates were subjected to pulsed-field gel electrophoresis (PFGE). Select isolates then underwent multilocus sequence typing (MLST). While the PFGE patterns tended to cluster within each hospital, sequence types (STs) belonging to the clonal complex 92 (CC92) and the pan-European clonal lineage II (EUII; worldwide clonal lineage 2) were predominant in all hospitals. Of them, ST122 and ST208 were the most common and were found in four of the six hospitals. Isolates belonging to the pan-European clonal lineages I and III were identified in one hospital each. Carbapenemase-encoding genes bla(OXA-23) and/or ISAba1-bla(OXA-51-like) were present among the majority of isolates. These findings suggest that carbapenem-nonsusceptible A. baumannii isolates found in U.S. hospitals constitute part of the global epidemic driven by CC92, but have unique STs other than ST92, which may be spreading by means of patient transfer between health care facilities within the United States.
鲍曼不动杆菌正在成为全球重要的医院感染病原体。我们报告了 2008 年至 2009 年间，在美国纽约、宾夕法尼亚、佛罗里达、密苏里、内华达和加利福尼亚的医院中发现的 65 株耐碳青霉烯鲍曼不动杆菌的分子流行病学。所有分离株均进行脉冲场凝胶电泳（PFGE）分析。然后选择部分分离株进行多位点序列分型（MLST）。PFGE 模式倾向于在每个医院内聚类，但属于克隆复合体 92（CC92）和泛欧克隆谱系 II（EUII；全球克隆谱系 2）的序列型（ST）在所有医院中均占主导地位。其中，ST122 和 ST208 最为常见，在六个医院中的四个医院中发现。泛欧克隆谱系 I 和 III 的分离株在每个医院中各发现一株。大多数分离株中存在碳青霉烯酶编码基因 bla(OXA-23)和/或 ISAba1-bla(OXA-51 样)。这些发现表明，美国医院中发现的耐碳青霉烯鲍曼不动杆菌分离株构成了由 CC92 驱动的全球流行的一部分，但具有不同于 ST92 的独特 ST，这可能是通过美国医疗机构之间的患者转移传播的。
