Department of Biochemistry, Kasturba Medical College, Manipal University, Manipal, 576104, Karnataka, India.
Neurochem Res. 2012 Feb;37(2):330-4. doi: 10.1007/s11064-011-0616-x. Epub 2011 Oct 1.
Oxidative stress is one of the common causes in etiopathogenesis of attention deficit hyperactivity disorder (ADHD). Hence, the salivary levels of protein thiols, ceruloplasmin, magnesium and pseudocholinesterase were estimated in children with ADHD. The symptoms of ADHD were identified using Conner's rating and DSM IV criteria. Saliva was collected and assessed for the levels of protein thiols, ceruloplasmin, magnesium and pseudocholinesterase, spectrophotometrically. It was also checked for pH and the flow rate was noted down. There was a significant increase (P < 0.001) in the salivary protein thiols and pseudocholinesterase levels in ADHD children when compared to controls. Ceruloplasmin levels did not show any significant change. Magnesium levels were significantly decreased (P < 0.001) in cases when compared to controls. Further, a receiver operating characteristic curve for validity of the biochemical parameters in saliva of ADHD children indicated a sensitivity and specificity above 90% for protein thiols and magnesium values. Our study shows that protein thiols, magnesium, and pseudocholinesterase might have a role in the pathogenesis of ADHD and saliva can be effectively used as a non-invasive tool for evaluation of such children.
氧化应激是注意缺陷多动障碍（ADHD）发病机制中的常见原因之一。因此，我们评估了 ADHD 儿童的唾液蛋白巯基、铜蓝蛋白、镁和拟胆碱酯酶水平。使用康纳评定量表和 DSM-IV 标准来确定 ADHD 的症状。采集唾液并使用分光光度法评估蛋白巯基、铜蓝蛋白、镁和拟胆碱酯酶的水平，同时检查唾液的 pH 值并记录流速。与对照组相比，ADHD 儿童的唾液蛋白巯基和拟胆碱酯酶水平显著升高（P<0.001），而铜蓝蛋白水平没有明显变化。与对照组相比，病例组的镁水平显著降低（P<0.001）。此外，ADHD 儿童唾液生化参数的受试者工作特征曲线表明，蛋白巯基和镁值的灵敏度和特异性均超过 90%。我们的研究表明，蛋白巯基、镁和拟胆碱酯酶可能在 ADHD 的发病机制中起作用，唾液可作为评估此类儿童的非侵入性工具。
