Michaels M L, Pham L, Nghiem Y, Cruz C, Miller J H
Molecular Biology Institute, University of California, Los Angeles 90024.
Nucleic Acids Res. 1990 Jul 11;18(13):3841-5. doi: 10.1093/nar/18.13.3841.
The mutY gene of Escherichia coli, which codes for an adenine glycosylase that excises the adenine of a G-A mispair, has been cloned and sequenced. The mutY gene codes for a protein of 350 amino acids (Mr = 39,123) and the clone genetically complements the mutY strain. The protein shows significant sequence homology to E. coli endonuclease III, an enzyme that has previously been shown to have glycosylase activity on damaged base pairs. Sequence analysis suggests that, like endonuclease III, MutY is an iron-sulfur protein with a [4Fe-4S]2+ cluster.
大肠杆菌的mutY基因已被克隆并测序，该基因编码一种腺嘌呤糖基化酶，可切除G-A错配中的腺嘌呤。mutY基因编码一个由350个氨基酸组成的蛋白质（Mr = 39,123），该克隆在遗传上可互补mutY菌株。该蛋白质与大肠杆菌内切核酸酶III具有显著的序列同源性，此前已证明该酶对受损碱基对具有糖基化酶活性。序列分析表明，与内切核酸酶III一样，MutY是一种含有[4Fe-4S]2+簇的铁硫蛋白。
