Kok T, Barancek K, Burrell C J
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
J Clin Microbiol. 1990 Jun;28(6):1458-9. doi: 10.1128/jcm.28.6.1458-1459.1990.
A premarket trial of the Becton Dickinson Directigen respiratory syncytial virus membrane-based enzyme immunoassay compared the test with virus isolation for the detection of respiratory syncytial virus in 583 nasopharyngeal aspirates. After modification, the Directigen test showed a sensitivity of 83% and a specificity of 90%. It offers the potential for an efficient bedside test--without the need for any equipment--for the diagnosis of respiratory syncytial virus infection and requires only a 0.25-ml sample volume. However, for optimum reliability, freezing-thawing of samples and access to a confirmatory test were shown to be necessary.
一项针对贝克顿·迪金森公司基于呼吸道合胞病毒膜的酶免疫测定法的上市前试验，将该检测方法与病毒分离法进行了比较，以检测583份鼻咽抽吸物中的呼吸道合胞病毒。经过改进后，迪瑞直检法显示出83%的灵敏度和90%的特异性。它具有进行高效床边检测的潜力——无需任何设备——用于诊断呼吸道合胞病毒感染，且仅需0.25毫升样本量。然而，为了获得最佳可靠性，样本的冻融处理以及进行确认性检测被证明是必要的。
