Ling J M, Zhang L C, Hui Y W, French G L
Department of Microbiology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories.
J Clin Pathol. 1990 Jul;43(7):587-90. doi: 10.1136/jcp.43.7.587.
The Cathra system is a commercial multipoint inoculation method for the identification of aerobic Gram negative bacteria. The system uses a replicator technique in which 21 different agar media can be inoculated simultaneously with 36 organisms. Identifications are made by use of a special computer database. The performance of this system was compared with that of the API 20E for the identification of 372 clinical isolates of Enterobacteriaceae and 133 miscellaneous Gram negative bacteria. For enterobacteria, the Cathra system was in 97% agreement with API 20E at species level and 98% at genus level. For miscellaneous Gram negative strains the two systems were in 59% agreement at species level and 77% at genus level. The Cathra system is suitable for use in diagnostic laboratories, especially those with a heavy workload and a wish to use break-point sensitivity testing. The identification database for miscellaneous Gram negative organisms, however, needs to be expanded.
Cathra系统是一种用于鉴定需氧革兰氏阴性菌的商业多点接种方法。该系统采用复制技术，可同时用36种微生物接种21种不同的琼脂培养基。通过使用特殊的计算机数据库进行鉴定。将该系统的性能与API 20E进行了比较，以鉴定372株临床分离的肠杆菌科细菌和133株其他革兰氏阴性菌。对于肠杆菌，Cathra系统在种水平上与API 20E的一致性为97%，在属水平上为98%。对于其他革兰氏阴性菌株，两个系统在种水平上的一致性为59%，在属水平上为77%。Cathra系统适用于诊断实验室，特别是那些工作量大且希望使用断点敏感性试验的实验室。然而，其他革兰氏阴性菌的鉴定数据库需要扩展。
