Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, People's Republic of China.
PLoS One. 2011;6(10):e26055. doi: 10.1371/journal.pone.0026055. Epub 2011 Oct 6.
Oxidative stress plays an important role in the pathogenesis of neurodegenerative diseases, such as Parkinson's disease. The molecule, 2,3,5,4'-tetrahydr- oxystilbene-2-O-β-D-glucoside (TSG), is a potent antioxidant derived from the Chinese herb, Polygonum multiflorum Thunb. In this study, we investigated the protective effect of TSG against 6-hydroxydopamine-induced apoptosis in rat adrenal pheochromocytoma PC12 cells and the possible mechanisms. Our data demonstrated that TSG significantly reversed the 6-hydroxydopamine-induced decrease in cell viability, prevented 6-hydroxydopamine-induced changes in condensed nuclei and decreased the percentage of apoptotic cells in a dose-dependent manner. In addition, TSG slowed the accumulation of intracellular reactive oxygen species and nitric oxide, counteracted the overexpression of inducible nitric oxide syntheses as well as neuronal nitric oxide syntheses, and also reduced the level of protein-bound 3-nitrotyrosine. These results demonstrate that the protective effects of TSG on rat adrenal pheochromocytoma PC12 cells are mediated, at least in part, by the ROS-NO pathway. Our results indicate that TSG may be effective in providing protection against neurodegenerative diseases associated with oxidative stress.
氧化应激在神经退行性疾病（如帕金森病）的发病机制中起着重要作用。这种名为 2,3,5,4'-四氢氧芪-2-O-β-D-葡萄糖苷（TSG）的分子是一种从中国草药何首乌中提取的强效抗氧化剂。在这项研究中，我们研究了 TSG 对大鼠肾上腺嗜铬细胞瘤 PC12 细胞中 6-羟多巴胺诱导的细胞凋亡的保护作用及其可能的机制。我们的数据表明，TSG 可显著逆转 6-羟多巴胺引起的细胞活力下降，防止 6-羟多巴胺引起的核浓缩变化，并以剂量依赖的方式降低凋亡细胞的百分比。此外，TSG 减缓了细胞内活性氧和一氧化氮的积累，拮抗诱导型一氧化氮合酶和神经元型一氧化氮合酶的过度表达，并降低了蛋白质结合的 3-硝基酪氨酸水平。这些结果表明，TSG 对大鼠肾上腺嗜铬细胞瘤 PC12 细胞的保护作用至少部分是通过 ROS-NO 途径介导的。我们的研究结果表明，TSG 可能对与氧化应激相关的神经退行性疾病具有保护作用。
