BK病毒DNA序列：与猴病毒40 DNA的同源程度。
BK virus DNA sequence: extent of homology with simian virus 40 DNA.
作者信息
Yang R C, Wu R
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1179-83. doi: 10.1073/pnas.76.3.1179.
The primary nucleotide sequence of three regions of BK virus (BKV) variant (MM) DNA has been determined. The region between map positions 0.715 and 0.900 includes the initiation points and partial coding sequences of the putative VP2 and VP3 proteins of BKV(MM), the amino acid sequences of which show over 80% homology with those of VP2 and VP3 of simian virus 40. The sequence of a potential leader protein X, 66 amino acids long for BKV(MM) and 62 long for simian virus 40, is also deduced. The regions between 0.595 and 0.398 and 0.310 and 0.175 include the coding sequence for the entire small t antigen and most of the large T antigen of BKV(MM). The DNA sequence within these regions comprises over 50% of the complete BKV(MM) genome and shows a 70% sequence homology with the corresponding regions of simian virus 40 DNA. This high degree of homology is at variance with the reported homology values of 11--20% estimated by hybridization measurements of heteroduplex analyses. Possible explanations for the discrepancy are discussed.
已确定BK病毒（BKV）变体（MM）DNA三个区域的初级核苷酸序列。图谱位置0.715至0.900之间的区域包括BKV（MM）假定的VP2和VP3蛋白的起始点和部分编码序列，其氨基酸序列与猿猴病毒40的VP2和VP3的氨基酸序列显示出超过80%的同源性。还推导了BKV（MM）潜在的前导蛋白X的序列，其长度为66个氨基酸，猿猴病毒40的该序列长度为62个氨基酸。0.595至0.398以及0.310至0.175之间的区域包括BKV（MM）整个小t抗原和大部分大T抗原的编码序列。这些区域内的DNA序列占完整BKV（MM）基因组的50%以上，并且与猿猴病毒40 DNA的相应区域显示出70%的序列同源性。这种高度同源性与通过异源双链分析的杂交测量估计的11%-20%的报道同源性值不一致。讨论了这种差异的可能解释。
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