[Determination of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in Usnea diffracta by RP-HPLC].

OBJECTIVE

To develop a RP-HPLC method for determining the contents of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in Usnea diffracta.

METHOD

A Kromasil-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phases of acetonitrile -1% acetic acid in a gradient manner. The flow rate was set at 1.0 mL x min(-1). The detection wavelength was 280 nm.

RESULT

The correlation coefficients of atranol, lecanorin, ethyl orsellinate, and methyl orsellinate were higher than 0.999. Recoveries were from 102.9% to 95.30%; with RSD from 2.3% to 1.9%.

CONCLUSION

The method is quick, simple and repeatable for simultaneous determination of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in U. diffracta.