长双歧杆菌亚种。婴儿 ATCC 15697 α-岩藻糖苷酶在岩藻糖基化的人乳低聚糖上具有活性。
Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides.
机构信息
Microbiology Graduate Group, University of California, Davis, Davis, California, USA.
出版信息
Appl Environ Microbiol. 2012 Feb;78(3):795-803. doi: 10.1128/AEM.06762-11. Epub 2011 Dec 2.
Abstract
Bifidobacterium longum subsp. infantis ATCC 15697 utilizes several small-mass neutral human milk oligosaccharides (HMOs), several of which are fucosylated. Whereas previous studies focused on endpoint consumption, a temporal glycan consumption profile revealed a time-dependent effect. Specifically, among preferred HMOs, tetraose was favored early in fermentation, with other oligosaccharides consumed slightly later. In order to utilize fucosylated oligosaccharides, ATCC 15697 possesses several fucosidases, implicating GH29 and GH95 α-L-fucosidases in a gene cluster dedicated to HMO metabolism. Evaluation of the biochemical kinetics demonstrated that ATCC 15697 expresses three fucosidases with a high turnover rate. Moreover, several ATCC 15697 fucosidases are active on the linkages inherent to the HMO molecule. Finally, the HMO cluster GH29 α-L-fucosidase possesses a crystal structure that is similar to previously characterized fucosidases.
摘要
长双歧杆菌亚种。婴儿 ATCC 15697 利用几种小分子量的中性人乳寡糖（HMOs），其中几种是岩藻糖基化的。虽然以前的研究集中在终点消耗上，但时间依赖性聚糖消耗谱揭示了一种时间依赖性效应。具体来说，在首选的 HMO 中，四糖在发酵早期被优先利用，而其他寡糖则稍晚被消耗。为了利用岩藻糖基化的寡糖，ATCC 15697 拥有几种岩藻糖苷酶，这暗示 GH29 和 GH95 α-L-岩藻糖苷酶在专门用于 HMO 代谢的基因簇中。生化动力学的评估表明，ATCC 15697 表达三种具有高周转率的岩藻糖苷酶。此外，几种 ATCC 15697 岩藻糖苷酶在 HMO 分子固有的键合上具有活性。最后，HMO 簇 GH29 α-L-岩藻糖苷酶具有与以前表征的岩藻糖苷酶相似的晶体结构。
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