Warkentin Matthew, Badeau Ryan, Hopkins Jesse B, Mulichak Anne M, Keefe Lisa J, Thorne Robert E
Physics Department, Cornell University, Ithaca, NY 14853, USA.
Acta Crystallogr D Biol Crystallogr. 2012 Feb;68(Pt 2):124-33. doi: 10.1107/S0907444911052085. Epub 2012 Jan 17.
Global radiation damage to 19 thaumatin crystals has been measured using dose rates from 3 to 680 kGy s⁻¹. At room temperature damage per unit dose appears to be roughly independent of dose rate, suggesting that the timescales for important damage processes are less than ∼1 s. However, at T = 260 K approximately half of the global damage manifested at dose rates of ∼10 kGy s⁻¹ can be outrun by collecting data at 680 kGy s⁻¹. Appreciable sample-to-sample variability in global radiation sensitivity at fixed dose rate is observed. This variability cannot be accounted for by errors in dose calculation, crystal slippage or the size of the data sets in the assay.
使用3至680 kGy s⁻¹的剂量率测量了19个奇异果甜蛋白晶体的整体辐射损伤。在室温下,单位剂量的损伤似乎大致与剂量率无关,这表明重要损伤过程的时间尺度小于约1秒。然而,在T = 260 K时,通过以680 kGy s⁻¹收集数据,可以避免在约10 kGy s⁻¹剂量率下表现出的约一半的整体损伤。在固定剂量率下,观察到整体辐射敏感性存在明显的样品间差异。这种差异不能用剂量计算误差、晶体滑移或测定中数据集的大小来解释。