Exercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sport Sciences at Serres, Aristotle University of Thessaloniki, Greece.
Biomarkers. 2012 Feb;17(1):28-35. doi: 10.3109/1354750X.2011.635805.
Twenty males ran either on a level treadmill (nonmuscle-damaging condition) or on a downhill treadmill (muscle-damaging condition). Blood and urine samples were collected before and after exercise (immediately after, 1h, 4h, 24h, 48h, and 96h). The following assays were performed: F(2)-isoprostanes in urine, protein carbonyls in plasma, glutathione, superoxide dismutase, glutathione peroxidase, and catalase in erythrocytes. The main finding was that monophasic redox responses were detected after nonmuscle-damaging exercise compared to the biphasic responses detected after muscle-damaging exercise. Based on these findings, muscle-damaging exercise may be a more appropriate experimental model to induce physiological oxidative stress.
20 名男性要么在水平跑步机上跑步（非肌肉损伤条件），要么在下坡跑步机上跑步（肌肉损伤条件）。在运动前后（运动后立即、1 小时、4 小时、24 小时、48 小时和 96 小时）采集血液和尿液样本。进行了以下检测：尿液中的 F(2)-异前列腺素、血浆中的蛋白质羰基、红细胞中的谷胱甘肽、超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶。主要发现是，与肌肉损伤运动后检测到的双相反应相比，非肌肉损伤运动后检测到单相氧化还原反应。基于这些发现，肌肉损伤运动可能是一种更合适的诱导生理氧化应激的实验模型。
