基质辅助激光解吸电离飞行时间质谱法鉴定临床相关棒状杆菌属、解鸟氨酸弧菌和马红球菌。
Identification of clinically relevant Corynebacterium spp., Arcanobacterium haemolyticum, and Rhodococcus equi by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
机构信息
Department of Clinical Microbiology, Hospital Clínic, CRESIB/IDIBAPS, School of Medicine, University of Barcelona, Barcelona, Spain.
出版信息
J Clin Microbiol. 2012 May;50(5):1745-7. doi: 10.1128/JCM.05821-11. Epub 2012 Feb 15.
Abstract
The identification of 83 Corynebacterium, 13 Arcanobacterium haemolyticum, and 10 Rhodococcus equi strains by conventional methods (API Coryne complemented with 16S rRNA gene sequence analysis) was compared with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry identification. The correlation between API and MALDI-TOF results was 89%. MALDI-TOF is a rapid and accurate system for identification of the above-mentioned microorganisms.
摘要
通过常规方法（API Coryne 补充 16S rRNA 基因序列分析）对 83 株棒状杆菌、13 株解血阿克曼菌和 10 株马红球菌进行鉴定，并与基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱鉴定进行比较。API 和 MALDI-TOF 结果之间的相关性为 89%。MALDI-TOF 是一种快速准确的系统，可用于鉴定上述微生物。
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