基质辅助激光解吸电离飞行时间质谱分析不属于链球菌属或肠球菌属的革兰阳性、触酶阴性球菌及数据库扩展的益处。
Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Gram-positive, catalase-negative cocci not belonging to the Streptococcus or Enterococcus genus and benefits of database extension.
机构信息
Department of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark.
出版信息
J Clin Microbiol. 2012 May;50(5):1787-91. doi: 10.1128/JCM.06339-11. Epub 2012 Mar 7.
Abstract
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in combination with a database extension generated in this study with 51 collection strains from 16 genera. Most strains were identified by using both databases individually, and some were identified only by applying the combined database. Thus, the methodology is very useful and the generated database extension was helpful.
摘要
基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱仪与 Bruker Daltonics microflex LT 系统联合应用于 90 株特征明确的过氧化氢酶阴性、革兰阳性球菌，这些菌不属于链球菌或肠球菌。Biotyper 版本 2.0.43.1 软件单独使用或与本研究中生成的数据库扩展联合使用，共包括来自 16 个属的 51 株采集株。大多数菌株通过单独使用两个数据库进行鉴定，一些菌株仅通过联合数据库鉴定。因此，该方法非常有用，生成的数据库扩展也很有帮助。
相似文献
1
Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Gram-positive, catalase-negative cocci not belonging to the Streptococcus or Enterococcus genus and benefits of database extension.基质辅助激光解吸电离飞行时间质谱分析不属于链球菌属或肠球菌属的革兰阳性、触酶阴性球菌及数据库扩展的益处。
J Clin Microbiol. 2012 May;50(5):1787-91. doi: 10.1128/JCM.06339-11. Epub 2012 Mar 7.
2
Comparison of direct colony method versus extraction method for identification of gram-positive cocci by use of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.直接菌落法与提取法在布鲁克生物梅里埃基质辅助激光解吸电离飞行时间质谱技术鉴定革兰阳性球菌中的比较。
J Clin Microbiol. 2011 Aug;49(8):2868-73. doi: 10.1128/JCM.00506-11. Epub 2011 May 25.
3
Identification of Gram-positive cocci by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry: comparison of different preparation methods and implementation of a practical algorithm for routine diagnostics.采用基质辅助激光解吸电离飞行时间质谱技术鉴定革兰阳性球菌：不同制备方法的比较及常规诊断实用算法的建立。
J Clin Microbiol. 2013 Jun;51(6):1834-40. doi: 10.1128/JCM.02654-12. Epub 2013 Apr 3.
4
Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories.对Vitek MS和Microflex LT这两种基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）系统进行比较评估，以鉴定临床微生物实验室常规分离出的革兰氏阳性球菌。
J Microbiol Methods. 2015 Jun;113:13-5. doi: 10.1016/j.mimet.2015.03.020. Epub 2015 Mar 25.
5
Identification of Gram-positive anaerobic cocci by MALDI-TOF mass spectrometry.利用 MALDI-TOF 质谱技术鉴定革兰阳性厌氧球菌。
Syst Appl Microbiol. 2011 Feb;34(1):58-62. doi: 10.1016/j.syapm.2010.11.005. Epub 2011 Feb 2.
6
Identification of clinical isolates of anaerobic bacteria using matrix-assisted laser desorption ionization-time of flight mass spectrometry.使用基质辅助激光解吸电离飞行时间质谱技术鉴定临床厌氧细菌分离株。
Eur J Clin Microbiol Infect Dis. 2012 Sep;31(9):2257-62. doi: 10.1007/s10096-012-1563-4. Epub 2012 Feb 28.
7
Species differentiation within the Staphylococcus intermedius group using a refined MALDI-TOF MS database.应用改良 MALDI-TOF MS 数据库进行中间葡萄球菌群的种内区分。
Clin Microbiol Infect. 2014 Oct;20(10):1007-15. doi: 10.1111/1469-0691.12662. Epub 2014 Jun 14.
8
MALDI-TOF mass spectrometry following short incubation on a solid medium is a valuable tool for rapid pathogen identification from positive blood cultures.在固体培养基上短暂培养后进行基质辅助激光解吸电离飞行时间质谱分析，是从阳性血培养物中快速鉴定病原体的一项重要工具。
Int J Med Microbiol. 2015 Jun-Aug;305(4-5):469-79. doi: 10.1016/j.ijmm.2015.04.004. Epub 2015 Apr 27.
9
Novel physico-chemical diagnostic tools for high throughput identification of bovine mastitis associated gram-positive, catalase-negative cocci.用于高通量鉴定与牛乳腺炎相关的革兰氏阳性、过氧化氢酶阴性球菌的新型物理化学诊断工具。
BMC Vet Res. 2014 Jul 11;10:156. doi: 10.1186/1746-6148-10-156.
10
Comparison of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to BD Phoenix automated microbiology system for identification of gram-negative bacilli.布鲁克 Biotyper 基质辅助激光解吸电离飞行时间质谱仪与 BD Phoenix 全自动微生物分析系统鉴定革兰氏阴性杆菌的比较。
J Clin Microbiol. 2011 Mar;49(3):887-92. doi: 10.1128/JCM.01890-10. Epub 2011 Jan 5.
引用本文的文献
1
The Expression Levels of Genes Responsible for the Enzymatic Activity of Bile Salt Hydrolase (BSH) and the Relationship of Cholesterol Assimilation in L. plantarum and L. paracasei.植物乳杆菌和副干酪乳杆菌中负责胆盐水解酶（BSH）酶活性的基因表达水平及其与胆固醇同化的关系。
Curr Microbiol. 2023 May 8;80(6):205. doi: 10.1007/s00284-023-03311-2.
2
Automatic Identification of MALDI-TOF MS Database Using Classical Species Isolates.基于经典分离株的 MALDI-TOF MS 数据库的自动鉴定。
Comput Math Methods Med. 2022 Jun 15;2022:1679951. doi: 10.1155/2022/1679951. eCollection 2022.
3
-Related Infections and their Significance: A 9-Year Retrospective Study.相关感染及其意义：一项9年回顾性研究。
J Microsc Ultrastruct. 2020 Nov 9;9(1):18-25. doi: 10.4103/JMAU.JMAU_61_19. eCollection 2021 Jan-Mar.
4
Network Analysis Based on Unique Spectral Features Enables an Efficient Selection of Genomically Diverse Operational Isolation Units.基于独特光谱特征的网络分析能够有效选择基因组多样化的操作隔离单元。
Microorganisms. 2021 Feb 17;9(2):416. doi: 10.3390/microorganisms9020416.
5
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based identification of security-sensitive bacteria: Considerations for Canadian Bruker users.基于基质辅助激光解吸/电离飞行时间质谱法的安全敏感细菌鉴定：加拿大布鲁克用户的注意事项
Can Commun Dis Rep. 2020 Oct 1;46(10):333-338. doi: 10.14745/ccdr.v46i10a04.
6
MALDI-TOF MS Supplementary database for species identification employing the yeast diversity encountered on southern Brazil grapes.用于利用巴西南部葡萄上发现的酵母多样性进行物种鉴定的基质辅助激光解吸电离飞行时间质谱补充数据库。
Folia Microbiol (Praha). 2018 Nov;63(6):685-693. doi: 10.1007/s12223-018-0607-2. Epub 2018 May 11.
7
, a rare pathogen that caused a skin abscess.一种导致皮肤脓肿的罕见病原体。
JMM Case Rep. 2018 Jan 16;5(2):e005137. doi: 10.1099/jmmcr.0.005137. eCollection 2018 Feb.
8
Clinical Characteristics and Laboratory Identification of Infections: An Australian Tertiary Centre Perspective.感染的临床特征与实验室鉴定：澳大利亚一家三级医疗中心的视角
Int J Microbiol. 2017;2017:5684614. doi: 10.1155/2017/5684614. Epub 2017 Sep 13.
9
An unusual case of thoracic empyema caused by (nutritionally variant streptococci) in a patient with pulmonary tuberculosis and human immunodeficiency virus infection.一名患有肺结核和人类免疫缺陷病毒感染的患者发生了由（营养变异链球菌）引起的罕见胸腔积脓病例。
JMM Case Rep. 2016 Oct 27;3(5):e005058. doi: 10.1099/jmmcr.0.005058. eCollection 2016 Oct.
10
Matrix-assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) as a Reliable Tool to Identify Species of Catalase-negative Gram-positive Cocci not Belonging to the Genus.基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）作为鉴定不属于该属的过氧化氢酶阴性革兰氏阳性球菌菌种的可靠工具。
Open Microbiol J. 2016 Dec 28;10:202-208. doi: 10.2174/1874285801610010202. eCollection 2016.
本文引用的文献
1
Application and use of various mass spectrometry methods in clinical microbiology.各种质谱方法在临床微生物学中的应用与使用。
Clin Microbiol Infect. 2010 Nov;16(11):1604-13. doi: 10.1111/j.1469-0691.2010.03368.x.
2
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry identifies 90% of bacteria directly from blood culture vials.基质辅助激光解吸电离飞行时间质谱法可直接从血培养瓶中鉴定 90%的细菌。
Clin Microbiol Infect. 2010 Nov;16(11):1631-8. doi: 10.1111/j.1469-0691.2010.03356.x.
3
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: usefulness for taxonomy and epidemiology.基质辅助激光解吸电离飞行时间质谱：在分类学和流行病学中的应用。
Clin Microbiol Infect. 2010 Nov;16(11):1626-30. doi: 10.1111/j.1469-0691.2010.03364.x.
4
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification.基质辅助激光解吸电离飞行时间质谱技术，临床微生物鉴定的一场革命。
Clin Microbiol Infect. 2010 Nov;16(11):1614-9. doi: 10.1111/j.1469-0691.2010.03311.x.
5
Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory.基质辅助激光解吸电离飞行时间质谱技术在常规临床微生物实验室分离的细菌菌株鉴定中的性能。
J Clin Microbiol. 2010 May;48(5):1549-54. doi: 10.1128/JCM.01794-09. Epub 2010 Mar 10.
6
Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level.比较两种基质辅助激光解吸电离飞行时间质谱法与传统表型鉴定方法，用于常规细菌种水平鉴定。
J Clin Microbiol. 2010 Apr;48(4):1169-75. doi: 10.1128/JCM.01881-09. Epub 2010 Feb 17.
7
High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories.常规医学微生物学实验室中基质辅助激光解吸电离飞行时间质谱法对细菌和酵母的高通量鉴定。
J Clin Microbiol. 2010 Mar;48(3):900-7. doi: 10.1128/JCM.02071-09. Epub 2010 Jan 6.
8
9
Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.细菌学领域正在进行的变革：通过基质辅助激光解吸电离飞行时间质谱法对细菌进行常规鉴定。
Clin Infect Dis. 2009 Aug 15;49(4):543-51. doi: 10.1086/600885.
10
Rapid identification of viridans streptococci by mass spectrometric discrimination.通过质谱鉴别快速鉴定草绿色链球菌
J Clin Microbiol. 2007 Aug;45(8):2392-7. doi: 10.1128/JCM.00556-07. Epub 2007 Jun 6.
Zotero 插件