Duke Eye Center, Durham, North Carolina, USA.
Invest Ophthalmol Vis Sci. 2012 Apr 6;53(4):1874-82. doi: 10.1167/iovs.11-9290.
This study was conducted to determine whether there were significant differences in choroidal thickness, contrast, outer choroidal vessel (OCV), and choroidal-scleral junction (CSJ) visualization in inverted versus upright spectral-domain optical coherence tomography (SD-OCT).
Images were captured on Bioptigen SD-OCT, Zeiss Cirrus HD-OCT, and Heidelberg Spectralis in 42 eyes of 21 healthy subjects. Average choroidal thickness across a fovea-centered 4-mm segment was determined with MATLAB. Quantitative measures of choroidal contrast were measured and CSJ assessed by applying a score of 0 to 3. OCV was determined by counting choroidal vessels ≥ 200 μm.
Mean choroidal thickness was greater in inverted versus upright images captured by Bioptigen (P ≤ 0.003) and Spectralis (P ≤ 0.015). Choroidal thickness varied significantly between the three machines (P < 0.05). Contrast was higher in inverted versus upright images captured by Bioptigen (P ≤ 0.02) and Spectralis (P < 0.001), but not in Cirrus (P > 0.10, both observers). CSJ score was highest in the following: Spectralis inverted = Spectralis EDI > Cirrus upright > Bioptigen inverted. Mean OCV was highest in Spectralis inverted mode.
The most favorable modes to visualize CSJ and OCV are the Spectralis EDI, Spectralis inverted, Cirrus upright, and Bioptigen inverted. These modes demonstrate the highest outer choroidal contrast and choroidal thickness measurements. Choroidal thickness cannot be compared between machines due to conversion factor differences. Future studies and construction of automated segmentation and detection software should take these benefits and pitfalls into account.
本研究旨在确定倒置与正立光谱域光学相干断层扫描（SD-OCT）相比，脉络膜厚度、对比、外脉络膜血管（OCV）和脉络膜-巩膜连接（CSJ）的可视化是否存在显著差异。
在 21 名健康受试者的 42 只眼中，使用 Bioptigen SD-OCT、Zeiss Cirrus HD-OCT 和 Heidelberg Spectralis 采集图像。使用 MATLAB 确定以黄斑为中心的 4mm 段的平均脉络膜厚度。通过应用 0 到 3 的评分来测量脉络膜对比度的定量测量值，并评估 CSJ。通过计数≥200μm 的脉络膜血管来确定 OCV。
Bioptigen（P≤0.003）和 Spectralis（P≤0.015）采集的倒置与正立图像的平均脉络膜厚度更大。三种机器之间的脉络膜厚度差异有统计学意义（P<0.05）。Bioptigen（P≤0.02）和 Spectralis（P<0.001）采集的倒置与正立图像的对比度更高，但 Cirrus 采集的图像对比度没有差异（P>0.10，两位观察者）。CSJ 评分最高的依次为：Spectralis 倒置=Spectralis EDI>Cirrus 正立>Bioptigen 倒置。Spectralis 倒置模式的平均 OCV 最高。
最有利于可视化 CSJ 和 OCV 的模式是 Spectralis EDI、Spectralis 倒置、Cirrus 正立和 Bioptigen 倒置。这些模式显示了最高的外脉络膜对比度和脉络膜厚度测量值。由于转换因子的差异，不同机器之间的脉络膜厚度无法进行比较。未来的研究和自动化分割和检测软件的构建应考虑到这些优势和缺陷。
