Kavli Institute of Nanoscience, Delft University of Technology, Delft, 2628 CJ, The Netherlands.
Annu Rev Biophys. 2012;41:453-72. doi: 10.1146/annurev-biophys-122311-100544. Epub 2012 Mar 14.
Since their first demonstration of manipulation and mechanical coiling of a single DNA molecule, magnetic tweezers have become a popular and widely used single-molecule manipulation technique. Magnetic tweezers have yielded a wealth of information on the elastic properties of (coiled) DNA and have provided critical insights into the dynamic activity of DNA-processing enzymes. Recent years have seen a wave of technological creativity, and a flurry of new methods and technological advances have been introduced to magnetic tweezers, which will enable more and previously inaccessible information to be extracted from biophysical systems. In this review, we survey the most important recent advances and reflect on more interesting twists to come.
自从首次展示对单个 DNA 分子的操纵和机械缠绕以来，磁镊已成为一种流行且广泛使用的单分子操纵技术。磁镊为（缠绕的）DNA 的弹性特性提供了丰富的信息，并为 DNA 加工酶的动态活性提供了重要的见解。近年来，技术创新浪潮涌现，许多新方法和技术进步被引入磁镊，这将使我们能够从生物物理系统中提取更多以前无法获取的信息。在这篇综述中，我们调查了最近最重要的进展，并思考了更多有趣的发展方向。
